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Further Characterization of Staphylococcal Gamma-Hemolysin

机译:葡萄球菌γ-溶血素的进一步表征

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It was confirmed that staphylococcal gamma-hemolysin is composed of two separate proteins (gamma-lysin components I and II) which act synergistically. The molecular weights of the two components, determined by gel filtration, are 29,000 and 26,000, respectively, and their isoelectric points, determined by isoelectric focusing, are at pH 9.8 and 9.9. Both components are susceptible to the action of Pronase and subtilisin. A wide range of lipids, some in minute amounts, are capable of inhibiting the hemolytic activity of gamma-lysin.
机译:证实了葡萄球菌的γ-溶血素由协同作用的两个单独的蛋白质(γ-溶素成分I和II)组成。通过凝胶过滤测定的两种组分的分子量分别为29,000和26,000,并且通过等电聚焦测定的它们的等电点为pH 9.8和9.9。两种成分都容易受到蛋白酶和枯草杆菌蛋白酶的作用。多种脂质(少量)可以抑制γ-溶素的溶血活性。

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