• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IFAC PapersOnLine >Multi-objective identification from fluorescence recovery after photobleaching experiments: Understanding morphogenetic regulation of epithelial polarity ?
【24h】

Multi-objective identification from fluorescence recovery after photobleaching experiments: Understanding morphogenetic regulation of epithelial polarity ?

机译：从光漂白实验后的荧光恢复中进行多目标鉴定：了解上皮极性的形态发生调控 ？

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many of the processes related to morphogenesis depend critically on the maintenance of the spatial distribution of certain polarity proteins on the plasma membrane of the cell. Maintenance of this epithelial polarity depends on the localization and concentration of the polarity proteins. The transmembrane protein Crumbs (Crb) is vital for the dimensions and integrity of the apical membrane. However, there is a little knowledge on the molecular mechanism controlling Crumbs dynamics during morphogenesis. Fluorescence recovery after photobleaching (FRAP) is a live cell imaging technique allowing for the functional measurement of protein mobility in living cells. FRAP experiments we performed in different parts of theDrosophila melanogasterembryonic epithelium and in different developmental stages show significant differences in their characteristics. We analyze the relationship of the changes in the FRAP experiments with the different morphogenetic states of the embryo. To this end, we develop a reaction-diffusion spatiotemporal model involving processes in the membrane and in the cytosol. Moreover, we model the fluorescent tags of proteins and the bleaching process to getin silicoFRAP experiments. Thesein silicoFRAPs are then used together with the experimental data to perform parameter estimation based on a multi-objective optimization design procedure. The multi-objective optimization is suitable to understand how morphogenetic events result in different sets of model parameters. Our parameter identification can reveal the mechanisms involved in the regulation of the Crb protein in the different stages of embryonic development and how morphogenesis affects these mechanisms.
机译：与形态发生有关的许多过程主要取决于某些极性蛋白在细胞质膜上的空间分布的维持。这种上皮极性的维持取决于极性蛋白的定位和浓度。跨膜蛋白碎屑（Crb）对于根尖膜的尺寸和完整性至关重要。但是，对形态发生过程中控制Crumbs动力学的分子机制知之甚少。光漂白后的荧光恢复（FRAP）是一种活细胞成像技术，允许对活细胞中蛋白质迁移率进行功能测量。我们在果蝇胚胎胚上皮的不同部位进行的FRAP实验以及在不同的发育阶段进行的FRAP实验显示出其特征的显着差异。我们分析了FRAP实验中变化与胚胎不同形态发生状态之间的关系。为此，我们开发了一种反应扩散时空模型，该模型涉及膜和胞质溶胶中的过程。此外，我们对蛋白质的荧光标签和漂白过程进行建模，以进行silicoFRAP实验。然后将这些insilicoFRAP与实验数据一起使用，以基于多目标优化设计程序进行参数估计。多目标优化适合于了解形态发生事件如何导致不同的模型参数集。我们的参数鉴定可以揭示在胚胎发育的不同阶段中调控Crb蛋白的机制，以及形态发生如何影响这些机制。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号