• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Ultrastructural Study of the Behavior of Macrophages Toward Parasitic Mycobacteria
【24h】

Ultrastructural Study of the Behavior of Macrophages Toward Parasitic Mycobacteria

机译：巨噬细胞对寄生性分枝杆菌行为的超微结构研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycobacterium lepraemurium and M. microti (causal agent of vole tuberculosis) were isolated from tissues of experimentally infected mice and used to infect normal mouse peritoneal macrophage cultures. The cellular response to these bacteria up to 4 days after infection was studied quantitatively by electron microscopy. Prelabeling with ferritin was used to facilitate observation of fusion between secondary lysosomes in the cells and phagosomes containing the bacteria. All bacteria were intraphagosomal, and a high proportion of them was morphologically “intact.” Nearly all phagosomes containing morphologically damaged (presumed nonviable) bacteria also contained ferritin, having fused with secondary lysosomes. Fusion of lysosomes had also occurred with most phagosomes containing intact M. lepraemurium but was infrequent with phagosomes containing intact M. microti. This tendency of multiplying mycobacteria of the tubercle type to avoid contact with lysosomal contents has already been reported for M. tuberculosis strain H37Rv. The different intracellular circumstances of the parasites may reflect different means of intracellular survival.
机译：麻风分枝杆菌和 M。从实验感染小鼠的组织中分离出小滴虫（田鼠结核的病原体），并用于感染正常小鼠腹膜巨噬细胞培养物。通过电子显微镜定量研究直至感染后4天对这些细菌的细胞应答。铁蛋白预标记用于促进观察细胞中次级溶酶体与含有细菌的吞噬体之间的融合。所有细菌都是吞噬体的，其中很大一部分在形态上是“完整的”。几乎所有含有形态受损（假定为不活细菌）细菌的吞噬体也含有铁蛋白，与次级溶酶体融合。溶酶体的融合也与大多数含有完整的 M的吞噬体发生了融合。麻风草，但很少含有完整的 M的吞噬体。 M已经报道了这种结节型分枝杆菌繁殖以避免与溶酶体含量接触的趋势。结核菌株H37Rv。寄生虫的不同细胞内情况可能反映了细胞内存活的不同方式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号