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首页> 外文期刊>Infection and immunity >Interactions Between Macrophages of Guinea Pigs and Salmonellae III. Bactericidal Action and Cytophilic Antibodies of Macrophages of Infected Guinea Pigs
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Interactions Between Macrophages of Guinea Pigs and Salmonellae III. Bactericidal Action and Cytophilic Antibodies of Macrophages of Infected Guinea Pigs

机译：豚鼠巨噬细胞和沙门氏菌之间的相互作用。感染豚鼠巨噬细胞的杀菌作用和亲细胞抗体

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The fate of virulent Salmonella typhimurium within macrophages of guinea pigs was assessed by a suspended cell culture procedure. The present study confirmed that macrophages of normal guinea pigs were capable of inactivating the ingested salmonellae. Macrophages of previously infected guinea pigs were not endowed with any significant increase in their ability to eliminate the ingested pathogen. However, the immune macrophages were observed to clump together tightly when they were exposed to salmonellae. This phenomenon was attributed to the presence of specific cytophilic antibodies on the immune macrophages. When immune macrophages were inactivated with Merthiolate, they agglutinated with both the H and the O antigens of S. typhimurium, but not with the O antigens of other species of Salmonella nor with the O antigens of Escherichia coli. Cytophilic antibodies could be eluted from immune macrophages by incubation in the absence of immune serum. Conversely, cytophilic antibodies could be passively transferred onto normal macrophages by incubation in the presence of immune serum. Furthermore, using immune serum previously adsorbed with the O antigens of S. typhimurium, cytophilic antibodies against the H antigens alone could be transferred onto normal macrophages, or those against the O antigens alone could be eluted from immune macrophages. These data suggest that immune macrophages possess specific cytophilic antibodies against both the H and the O antigens of S. typhimurium. It is proposed that the presence of cytophilic antibodies on immune macrophages represents an expression of antibacterial cellular immunity by enhanced clumping and phagocytic activities of the macrophages.
机译：通过悬浮细胞培养程序评估豚鼠巨噬细胞内强毒鼠伤寒沙门氏菌的命运。本研究证实正常豚鼠的巨噬细胞能够使摄入的沙门氏菌失活。先前感染过的豚鼠的巨噬细胞在消除被摄入的病原体的能力方面并未获得任何显着提高。但是，观察到免疫巨噬细胞暴露于沙门氏菌时会紧密结块。这种现象归因于免疫巨噬细胞上存在特定的嗜细胞抗体。当免疫巨噬细胞被硫醇盐灭活时，它们会与 S的H和O抗原凝集。鼠伤寒沙门氏菌，但不能与其他沙门氏菌沙门氏菌的O抗原或大肠杆菌 E.scherichia coli的 O抗原结合。在没有免疫血清的情况下。相反，通过在免疫血清的存在下孵育，可以将嗜细胞抗体被动转移到正常的巨噬细胞上。此外，使用预先吸附了 S的O抗原的免疫血清。鼠伤寒抗体可以将单独的针对H抗原的嗜细胞抗体转移到正常的巨噬细胞上，也可以将针对O抗原的嗜细胞抗体从免疫巨噬细胞上洗脱下来。这些数据表明免疫巨噬细胞具有针对 S的H和O抗原的特异的嗜细胞抗体。有人提出，免疫巨噬细胞上亲细胞抗体的存在可以通过增强巨噬细胞的聚集和吞噬活性来表达抗菌细胞免疫的表达。

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