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首页> 外文期刊>Infection and immunity >Tissue Receptor for Cholera Exotoxin: Postulated Structure from Studies with GM1 Ganglioside and Related Glycolipids
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Tissue Receptor for Cholera Exotoxin: Postulated Structure from Studies with GM1 Ganglioside and Related Glycolipids

机译：霍乱外毒素的组织受体：GM1神经节苷脂和相关糖脂研究的假定结构。

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摘要

By a double-diffusion precipitation-in-gel technique, isolated cholera toxin as well as its natural toxoid were shown to be fixed and precipitated by the ganglioside GM1 but not by any of the related glycolipids GM3, GM2, GM1-GlcNAc, GD1a, GD1b, GT1, globoside, GA1, and tetrahexoside-GlcNAc. Twenty-five nanograms of GM1 was enough to give a precipitation line with 1.2 μg of toxin, whereas about 50 ng was required with this amount of toxoid. GM1 also inactivated the toxin in the ileal loop as well as in the intradermal models in rabbits. A 1: 1 molar ratio of ganglioside to toxin was found limiting, e.g., 100 pg of GM1 could inactivate 5 ng (about 50 blueing doses) of isolated toxin. GM1 inactivated crude toxin (culture fil rate) with the same efficiency as isolated toxin, and the inactivating capacity of GM1 was unaffected by mixing with other gangliosides, indicating the specificity in the reaction between GM1 and toxin. The other glycolipids tested did not inactivate toxin except GD1a and GA1 which did so with approximately 1,000 times less efficiency than GM1. This identified the portion Gal → GalNAc [Formula: see text] as the critical region in GM1 for toxin fixation, and it is postulated that this may be the tissue receptor structure for the cholera toxin.
机译：通过凝胶中的双扩散沉淀技术，分离出的霍乱毒素及其天然类毒素被神经节苷脂G M1 固定并沉淀，但没有被相关的糖脂G < sub> M3 ，G M2 ，G M1 -GlcNAc，G D1a ，G D1b ， G T1 ，globoside，G A1 和四己糖苷-GlcNAc。 25纳克的G M1 足以产生一条含1.2μg毒素的沉淀线，而这种量的类毒素需要约50 ng。 G M1 还能使家兔回肠环以及皮内模型中的毒素失活。发现神经节苷脂与毒素的摩尔比为1：1，例如100 pg的G M1 可以灭活5 ng（约50份发蓝剂量）的分离毒素。 G M1 灭活的粗毒素（培养物过滤速率）与分离毒素相同，并且与其他神经节苷脂混合后，G M1 的灭活能力不受影响。 G M1 与毒素之间的反应。除G D1a 和G A1 效率比G M1 低约1,000倍外，其他测试的糖脂均未使毒素失活。这就确定了Gal→GalNAc [公式：见正文]部分是G M1 中毒素固定的关键区域，并推测这可能是霍乱毒素的组织受体结构。

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