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首页> 外文期刊>Infection and immunity >Thymidine kinaseless revertants of Ltk- cells transformed by herpes simplex virus type 1 are resistant to retransformation by homologous virus.
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Thymidine kinaseless revertants of Ltk- cells transformed by herpes simplex virus type 1 are resistant to retransformation by homologous virus.

机译：由1型单纯疱疹病毒转化的Ltk细胞的无胸苷激酶还原剂对同源病毒的再转化具有抗性。

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摘要

Mouse L cells lacking thymidine kinase (Ltk-) that had been transformed to the thymidine kinase-positive (tk+) phenotype by herpes simplex virus type 1 (HSV-1) were cultured in medium containing tritiated thymidine. Six clonal lines of cells surviving this treatment were found to have the following properties: (i) the cells were tk- and had spontaneous back-reversion frequencies to the tk+ phenotype of 10(-5) or less, (ii) the cells contained HSV antigens, although in lesser amounts than in the parental transformed cells, and (iii) the cells were retransformable to the tk+ phenotype by HSV-1 at frequencies of about 1 to 13% of the frequency of the primary transformation of LtK- cells. HSV-1 plaqued as efficiently on monolayers of these cells and replicated in them to the same extent as it did in Ltk- cells. These results indicate that HSV-1-transformed L cells surviving selection with tritiated thymidine are unlike the parental Ltk- cells in that they are damaged in such a way that the cells are resistant to retransformation by homologous virus, although they remain fully permissive for virus replication.
机译：将不含胸苷激酶（Ltk-）的小鼠L细胞（已被单纯疱疹病毒1型（HSV-1）转化为胸苷激酶阳性（tk +）表型）培养在含tri化胸苷的培养基中。幸存的六种克隆细胞系具有以下特性：（i）这些细胞为tk-型，并自发回复到tk +表型的频率为10（-5）或更低，（ii）所含细胞HSV抗原的数量虽然少于亲本转化细胞的数量，但（iii）该细胞可通过HSV-1在LtK细胞初次转化频率的约1％至13％的频率下重新转化为tk +表型。 HSV-1在这些细胞的单层上有效斑块，并在其中复制，复制程度与Ltk细胞相同。这些结果表明，在用ated化胸腺嘧啶核苷选择后存活的HSV-1转化L细胞与亲本Ltk细胞不同，它们被破坏的方式是细胞对同源病毒具有抗性，尽管它们仍然完全允许病毒传播。复制。

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