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首页> 外文期刊>Infection and immunity >Cholesteryl de-esterifying enzyme from Staphylococcus aureus: separation from alpha toxin, purification, and some properties.
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Cholesteryl de-esterifying enzyme from Staphylococcus aureus: separation from alpha toxin, purification, and some properties.

机译：金黄色葡萄球菌的胆固醇去酯化酶：与α毒素的分离，纯化和某些特性。

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A cholesteryl de-esterifying enzyme found in partially purified preparations of alpha toxin produced by the Wood 46 strain on Staphylococcus aureus has been separated from other staphylococcal proteins and from alpha toxin by isoelectric focusing and gel filtration. Preparations of alpha toxin from Bi-Gel P-60 columns and of the cholesteryl esterase from Bio-Gel P-200 columns showed a high degree of purity, as determined by analytical ultracentrifugation, gel diffusion, immunoelectrophoresis, and polyacrylamide gel electrophoresis. The molecular weight of the cholesteryl esterase determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate was 25,500 and on Bio-Gel P-300 columns it was 175,000, indicating an associating system. The density of the enzyme was lower than expected for simple proteins (about 1.19 g/ml). Chloroform-methanol extracts showed the presence of a neutral lipid that did not contain cholesterol. This material, possibly a glycolipid, might play a role in the stabilization of the enzymatically active protomer. The isoelectric point of the esterase was 9.1. Cholesteryl esterase was labile and lost its activity easily. It could bind reversibly to agarose-containing gels. After elution, it was enzymatically inactive, with an isoelectric point of less than 6.2. The W46M mutant of the Wood 46 strain, which does not produce alpha toxin, also does not produce cholesteryl esterase.
机译：通过等电聚焦和凝胶过滤，在由金黄色葡萄球菌Wood 46菌株产生的部分纯化的α毒素制剂中发现的胆甾醇脱酯酶已与其他葡萄球菌蛋白和α毒素分离。通过分析超速离心，凝胶扩散，免疫电泳和聚丙烯酰胺凝胶电泳确定，Bi-Gel P-60色谱柱制备的α毒素和Bio-Gel P-200色谱柱制备的胆固醇酯酶具有很高的纯度。通过聚丙烯酰胺凝胶电泳在十二烷基硫酸钠中测定的胆固醇酯酶的分子量为25,500，在Bio-Gel P-300色谱柱上的分子量为175,000，表明有缔合体系。该酶的密度低于简单蛋白质的预期值（约1.19 g / ml）。氯仿-甲醇提取物显示存在不含胆固醇的中性脂质。这种物质（可能是糖脂）可能在酶促活性原核蛋白的稳定中发挥作用。酯酶的等电点为9.1。胆固醇酯酶不稳定并且容易丧失其活性。它可以可逆地结合到含琼脂糖的凝胶上。洗脱后，它是无酶活性的，等电点小于6.2。 Wood 46菌株的W46M突变体不产生α毒素，也不产生胆固醇酯酶。

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