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首页> 外文期刊>Infection and immunity >Cellular streptolysin S-related hemolysins of group A Streptococcus C203S.
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Cellular streptolysin S-related hemolysins of group A Streptococcus C203S.

机译：A组链球菌C203S的细胞链球菌溶血素S相关溶血素。

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Group A streptococci strain C203S, grown in heart infusion broth with 0.3% maltose, produce two cellular hemolysins related to extracellular streptolysin S (SLS). Enzymatic lysis of the streptococci by group C streptococcal phage-associated lysin results in release of low titer, labile hemolysin, which can be stabilized by ribonucleic acid (RNA)-core (RNA preparation from yeast). This labile hemolysin can be detected only after the higher titer cellular streptolysin O is removed by erythrocyte membranes or inactivated by N-ethylmaleimide. The other cellular SLS-related hemolysin is released in a latent state (potential hemolysin) which can be activated to high-titer hemolysin by sonication with RNA-core. The titer of such activated hemolysin depends upon the intensity of sonic energy, duration of sonication, and amount of RNA-core. RNA obtained from the streptococci is far less effective than RNA-core. When the cocci are disrupted by sonication or grinding, potential hemolysin and/or activated form may be released, depending upon the conditions employed. The potential hemolysin material is large and heterogeneous; activation appears to involve, in part, disaggregation or fragmentation. Labile hemolysin, potential hemolysin, and the activated form of potential hemolysin can all be converted to hemolysin having the same hemolytic and physical properties as RNA-core SLS, suggesting that all have the same hemolytic moiety. The presence of glucose in heart infusion broth prevents formation of both potential hemolysin and RNA-core SLS by log-phase cells, whereas addition of glucose to a culture in heart infusion broth with 0.3% maltose stops accumulation of potential hemolysin but does not affect continuation of RNA-core SLS release. These results suggest that potential hemolysin is a cellular precursor to RNA-core SLS.
机译：在含0.3％麦芽糖的心脏输液肉汤中生长的A组链球菌菌株C203S，产生两种与细胞外链球菌溶血素S（SLS）相关的细胞溶血素。 C组链球菌噬菌体相关的溶素对链球菌的酶解作用导致释放低滴度，不稳定的溶血素，可通过核糖核酸（RNA）核心（从酵母中制备RNA）稳定该溶血素。仅在较高滴度的细胞链球菌溶血素O被红细胞膜去除或被N-乙基马来酰亚胺灭活后，才能检测到这种不稳定的溶血素。其他与细胞SLS相关的溶血素以潜在状态释放（潜在的溶血素），可通过与RNA核心进行超声处理将其激活为高滴度溶血素。这种活化的溶血素的效价取决于声能的强度，声处理的持续时间以及RNA核心的数量。从链球菌获得的RNA远不如RNA核心有效。当通过超声或研磨破坏球菌时，取决于所采用的条件，可能释放出潜在的溶血素和/或活化形式。潜在的溶血素物质很大且不均一;激活似乎部分涉及分解或破碎。不稳定的溶血素，潜在的溶血素和活化形式的潜在的溶血素都可以被转化为具有与RNA核心SLS相同的溶血和物理特性的溶血素，表明它们都具有相同的溶血部分。心脏输液肉汤中存在葡萄糖可防止对数期细胞形成潜在的溶血素和RNA核心SLS，而向含有0.3％麦芽糖的心脏输液肉汤中添加葡萄糖可阻止潜在的溶血素积聚，但不影响持续性RNA核心SLS的释放。这些结果表明潜在的溶血素是RNA核心SLS的细胞前体。

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