...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Detection of long-term cellular immunity to Coxiella burneti as assayed by lymphocyte transformation.
【24h】

Detection of long-term cellular immunity to Coxiella burneti as assayed by lymphocyte transformation.

机译：通过淋巴细胞转化测定对伯氏柯氏杆菌的长期细胞免疫的检测。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Delayed hypersensitivity to the antigens of Coxiella burneti, Nine Mile strain, was demonstrated in human subjects with various past histories of exposure to the organism by using lymphocyte transformation assays. Individuals with histories indicating exposure to C. burneti up to 8 years before the study demonstrated marked lymphocyte transformation in vitro to whole-cell antigens consisting of formalin-killed C. burneti phase I and phase II. These individuals also demonstrated a marked lymphocyte response to the trichloracetic acid-soluable phase I antigen. One individual who acquired Q fever during the study and one individual who received an experimental Q fever vaccine 4 years earlier were also evaluated by the lymphocyte transformation assay. It was also found that phase I trichloroacetic acid-soluble material was capable of acting as an antigen in the assay, whereas the phase II trichloroacetic acid-soluble material did not contain any antigenic material capable of causing lymphocyte transformation. The complete phase I trichloroacetic acid-soluble antigen, which was found to consist of protein and carbohydrate, was chemically fractionated into monospecific fractions. The fraction treated to eliminate carbohydrate was the only fraction found to elicit an in vitro response.
机译：通过使用淋巴细胞转化试验，在过去有多种暴露于该生物体的历史的人类受试者中证明了对九英里菌株伯氏柯氏杆菌抗原的迟发型超敏反应。有研究表明暴露于伯氏梭状芽胞杆菌长达8年的历史个体在体外显示出明显的淋巴细胞转化为由福尔马林杀死的伯氏梭状芽胞杆菌I期和II期组成的全细胞抗原。这些个体还表现出对三氯乙酸可溶的I相抗原的明显淋巴细胞反应。还通过淋巴细胞转化测定法评估了在研究期间获得Q发热的个体和4年前接受实验性Q发热疫苗的个体。还发现I相三氯乙酸可溶物质能够在测定中充当抗原，而II相三氯乙酸可溶物质不包含任何能够引起淋巴细胞转化的抗原物质。发现由蛋白质和糖类组成的完整的I相三氯乙酸可溶抗原被化学分离成单特异性组分。经处理以消除碳水化合物的馏分是唯一发现可引起体外反应的馏分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号