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首页> 外文期刊>Infection and immunity >Biochemical and immunological properties of ribonucleic acid-rich extracts from Francisella tularensis.
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Biochemical and immunological properties of ribonucleic acid-rich extracts from Francisella tularensis.

机译：图拉尼斯弗朗西斯菌富含核糖核酸的提取物的生化和免疫学特性。

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摘要

Ribonucleic acid (RNA)-rich extracts derived from the attenuated strain of Francisella tularensis (strain LVS) protected Swiss mice against lethal challenge with F. tularensis strain 425 but not against strain SCHU S4. No killed preparation, including an RNA-rich extract from SCHU S4 itself, offered protection against strain SCHU S4 in contrast to the high level of protection offered against this strain by vaccination with live strain LVS. The protective activity observed against strain 425 was sensitive to ribonuclease but not to Pronase. Protective activity is not a general property of bacterial RNA, since RNA-rich extracts from Staphylococcus aureus offered no protection against tularemia, although disc gel electrophoresis showed similar kinds and amounts of RNA in preparations form F. tularensis and S. aureus. Furthermore, inability to localize activity to a specific region in sucrose gradients suggests a structural rather than an informational role for the RNA in such extracts. RNA-rich extracts from F. tularensis but not from S. aureus were efficient inducers of F. tularensis opsonins in mouse serum, suggesting one mechanism by which such extracts confer protection.
机译：来自土拉弗朗西斯菌减毒株（LVS株）的富含核糖核酸（RNA）的提取物可保护瑞士小鼠免受土拉弗朗西斯菌425株的致死性攻击，但不能抵抗SCHU S4株。与活疫苗LVS疫苗接种所提供的针对该菌株的高水平保护相比，没有杀死的制剂（包括来自SCHU S4本身的富含RNA的提取物）提供对SCHU S4菌株的保护。观察到的针对菌株425的保护活性对核糖核酸酶敏感但对链霉蛋白酶不敏感。保护活性不是细菌RNA的一般特性，因为来自金黄色葡萄球菌的富含RNA的提取物没有提供抗Tularemia的保护作用，尽管圆盘凝胶电泳显示F. tularensis和S. aureus制剂中的RNA种类和数量相似。此外，无法将活性定位于蔗糖梯度中的特定区域提示这种提取物中RNA的结构作用而不是信息作用。来自土拉弗朗西斯菌而不是来自金黄色葡萄球菌的富含RNA的提取物是小鼠血清中土拉弗朗西斯菌调理素的有效诱导剂，表明这种提取物赋予保护作用的一种机制。

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