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首页> 外文期刊>Infection and immunity >Susceptibility of thymectomized and irradiated mice to challenge with several organisms and the effect of dapsone on infection with Mycobacterium leprae.
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Susceptibility of thymectomized and irradiated mice to challenge with several organisms and the effect of dapsone on infection with Mycobacterium leprae.

机译：经胸腺切除和辐照的小鼠易受几种生物攻击，并有氨苯砜对麻风分枝杆菌感染的影响。

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B6C3F1 mice that had been thymectomized at 8 to 12 weeks of age, subjected to 950 R of whole-body X irradiation, and transfused with syngeneic bone marrow were challenged in a footpad with Mycobacterium leprae or M. marinum, or intravenously or intraperitioneally with Listeria monocytogenes. Also, mice inoculated with M. leprae in a hind footpad were administered dapsone in the mouse chow. The thymectomized-irradiated (T + R) mice did not survive as well as non-thymectomized mice when housed in the vivarium with no special precautions, but survived sufficiently well to permit the completion of some long-term experiments. M. leprae multiplied to a higher "ceiling" and survived longer in the T + R mice than in the non-thymectomized controls. But a ceiling to multiplication of M. leprae was imposed, and finally the organisms were killed. The histopathological appearance of the footpad tissues, studied by electron microscopy, was consistent with the measurements of bacterial numbers and viability. Swelling of the footpad after local inoculation with M. marinum was greater in T + R mice than in non-thymectomized controls. Similarly, the number of L. monocytogenes following intravenous challenge was greater in the spleens of T + R than of non-thymectomized mice, and the survival of the T + R mice was impaired after intraperitoneal challenge with L.monocytogenes, compared to the survival of non-thymectomized mice. None of these differences was striking, suggesting that these T + R mice had retained or regained some immune competence. The effects of dapsone treatment of T + R mice inoculated with M. leprae were much the same as those of treatment of non-thymectomized mice. Because these T + R mice were not greatly immunosuppressed, they would not have provided a model of human lepromatous leprosy suitable for chemotherapeutic studies.
机译：将在8至12周龄时进行胸腺切除术，接受950 R全身X辐射并输注同基因骨髓的B6C3F1小鼠在脚垫中接种麻风分枝杆菌或海藻支原体，或静脉内或腹膜内接种李斯特菌单核细胞增生而且，在后足垫中接种了麻风分枝杆菌的小鼠在小鼠食物中被给予氨苯砜。经胸腺切除的（T + R）小鼠在没有特殊预防措施的情况下没有活在未经胸腺切除的小鼠中，但存活得很好，可以完成一些长期实验。与非经胸腺切除的对照组相比，麻风分枝杆菌在T + R小鼠中繁殖出更高的“上限”，并且存活时间更长。但是强加​​了麻风杆菌的繁殖上限，最终杀死了这些生物。通过电子显微镜研究，脚垫组织的组织病理学外观与细菌数量和生存力的测量结果一致。在T + R小鼠中，局部接种海藻支原体后，脚垫的肿胀比未经胸腺切除术的对照组要大。同样，在静脉内攻击后，T + R脾脏中的单核细胞增生李斯特菌的数量比未经胸腺切除的小鼠要多，并且与单核细胞增生李斯特菌相比，腹膜内攻击后T + R小鼠的生存受到损害。非经胸腺切除的小鼠。这些差异均无惊人之处，表明这些T + R小鼠已保留或恢复了一定的免疫能力。氨苯砜对麻疯分枝杆菌接种的T + R小鼠的治疗效果与未经胸腺切除的小鼠的治疗效果非常相似。由于这些T + R小鼠并未受到很大的免疫抑制，因此它们不会提供适合化学疗法研究的人类麻风麻风病模型。

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