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首页> 外文期刊>Infection and immunity >Detection of hepatitis A antigen by immunofluorescence.
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Detection of hepatitis A antigen by immunofluorescence.

机译：通过免疫荧光检测甲型肝炎抗原。

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Hepatitis A antigen (HA Ag) was demonstrated by immunofluorescence (IF) in liver biopsies from chimpanzees with experimental hepatitis A virus infection. Blocking experiments with paired sera from patients with hepatitis types A, B, or non-A, non-B, as well as with purified HA Ag, showed that the fluorescence was specific for HA Ag. HA Ag could be demonstrated only in biopsies from chimpanzees inoculated with hepatitis A virus. In two of four chimpanzees biopsied weekly, HA Ag could be detected by IF before stool shedding of HA Ag, elevation in serum alanine aminotransferase (SGPT), or histopathological evidence of liver disease was seen. The HA Ag was detected for 4 to 5 weeks; the last IF-positive biopsy was obtained after SGPT activity had returned to normal. In the two other chimpanzees, HA Ag could be detected only in the biopsy taken at the time of SGPT elevation. In the early IF-positive biopsies, HA Ag was diffusely distributed in the cytoplasm of many cells, but it later accumulated in a focal distribution in the cytoplasm of a few of the hepatocytes and Kupffer cells. This cytoplasmic distribution agrees with previous electron microscopic data.
机译：在患有实验性甲型肝炎病毒感染的黑猩猩的肝脏活检中，通过免疫荧光（IF）证实了甲型肝炎抗原（HA Ag）。用A型，B型或非A型，非B型肝炎患者的成对血清以及纯化的HA Ag进行的阻断实验表明，荧光对HA Ag具有特异性。只有在接种甲型肝炎病毒的黑猩猩的活组织检查中才能证明HA Ag。在每周进行活检的四只黑猩猩中，有两只在粪便脱落，血清丙氨酸氨基转移酶（SGPT）升高或肝脏疾病的组织病理学证据出现之前，可以通过IF检测到HA Ag。检测到HA Ag持续4至5周; SGPT活动恢复正常后，最后一次IF阳性活检。在其他两只黑猩猩中，只有在SGPT升高时进行的活检中才能检测到HA Ag。在早期的IF阳性活检中，HA Ag分散地分布在许多细胞的细胞质中，但后来又聚集在一些肝细胞和Kupffer细胞的细胞质中。这种细胞质分布与先前的电子显微镜数据一致。

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