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首页> 外文期刊>Infection and immunity >Immune response in rabbits to virion and nonvirion antigens of the Flavivirus kunjin.
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Immune response in rabbits to virion and nonvirion antigens of the Flavivirus kunjin.

机译：兔对黄病毒昆昆病毒颗粒和非病毒颗粒抗原的免疫反应。

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The nature of the antibodies formed in rabbits in response to the following Kunjin virus antigens was examined: infectious suckling mouse brain (SMB), purified virion or rapidly sedimenting hemagglutinin (RHA), slowly sedimenting hemagglutinin (SHA), and envelope fragments prepared from RHA disrupted by 0.1 or 0.2% sodium deoxycholate (DOC). The hemagglutination-inhibiting (HI) and neutralizing antibody responses to SMB, RHA, and large envelope fragments (0.1% DOC) were remarkably uniform, antibodies appearing at the same time, attaining similar HI titers (lowest to envelope), and being of similar avidity early and late in the respone. The 19S (immunoglobulin M) antibodies to all antigens were always relatively type-specific, whereas the 7S (immunoglobulin G) antibodies were always broadly cross-reactive in HI tests. These results confirm that the envelope antigen is the principal antigen involved in the stimulation of protective neutralizing antibodies and contains both type- and group-specific antigenic determinants. The results also establish that there is no significant advantage in using purified RHA or SHA either for immunization or as hemagglutinin antigens in attempts to obtain greater specificity in the HI test. No differences were detected in the antibody responses to infective Kunjin virus, within the range 1,400 to 10(9) plaque-forming units (PFU). Below 1,400 PFU, there was no detectable response. Inactivated virus (10(6) PFU) also stimulated the normal antibody response. In contrast, small envelope fragments (derived with 0.2% DOC) and a detergent-solubilized extract of infected cells were unable to stimulate a detectable antibody response and the small envelope fragments may have induced low dose tolerance in one of two rabbits.
机译：检查了对以下昆金病毒抗原的反应在兔中形成的抗体的性质：感染性乳鼠脑（SMB），纯化的病毒体或快速沉降的血凝素（RHA），缓慢沉降的血凝素（SHA）和由RHA制备的包膜片段被0.1或0.2％脱氧胆酸钠（DOC）破坏。对SMB，RHA和大包膜片段（0.1％DOC）的血凝抑制（HI）和中和抗体反应非常均匀，抗体同时出现，获得相似的HI滴度（最低至包膜），并且相似在响应的早期和晚期都具有热情。针对所有抗原的19S（免疫球蛋白M）抗体始终是相对类型特异性的，而7S（免疫球蛋白G）抗体在HI测试中始终具有广泛的交叉反应性。这些结果证实，包膜抗原是参与保护性中和抗体刺激的主要抗原，并且包含类型特异性抗原决定簇和组特异性抗原决定簇。结果还证实，使用纯化的RHA或SHA进行免疫或作为血凝素抗原在HI试验中获得更大的特异性方面没有显着优势。在对感染性昆金病毒的抗体反应中，在1400至10（9）噬菌斑形成单位（PFU）范围内未检测到差异。低于1,400 PFU，没有可检测到的响应。灭活的病毒（10（6）PFU）也刺激了正常的抗体反应。相反，小包膜碎片（衍生自0.2％DOC）和去污剂增溶的感染细胞提取物不能刺激可检测的抗体反应，并且小包膜碎片可能在两只兔子之一中诱导了低剂量耐受性。

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