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首页> 外文期刊>Infection and immunity >Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assays and defined toxoplasmosis serological patterns.
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Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assays and defined toxoplasmosis serological patterns.

机译：免疫球蛋白G和免疫球蛋白M酶联免疫吸附测定和确定的弓形虫血清学模式。

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Enzyme-linked immunosorbent assay (ELISA) for toxoplasmosis was evaluated in serum samples presenting defined toxoplasmosis serological patterns, as determined by results in immunoglobulin (Ig)G and IgM immunofluorescence (IgG-IF, IgM-IF), hemagglutination, and complement fixation tests. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Serum titer was expressed as the serum dilution end point determined by mere observation of color development in the test. A straight agreement was found between IgG-ELISA and IgG-IF titers, both in group A sera of ancient infections (patterns II and III) and group B sera of recent infections (pattern I). A similar agreement was found between IgG-ELISA and hemagglutination titers in group A sera, for which coincident IgG-IF and hemagglutination titers are also frequent. However, in group B sera, in spite of the same toxoplasma extract being used to sensitize both plastic surfaces and erythrocytes, IgG-ELISA titers were much higher than hemagglutination titers, in a way similar to that observed for IgG-IF titers. IgM-ELISA was positive in every group B serum, with higher titers than corresponding IgM-IF titers. Occasional low-titered positive IgM-ELISA results were seen for group A sera, sometimes due to IgM-antiglobulin antibodies. An easy test to perform, ELISA seems to be an adequate substitute for toxoplasmosis IF tests for routine purposes.
机译：通过血清免疫球蛋白（Ig）G和IgM免疫荧光（IgG-IF，IgM-IF），血凝和补体固定试验的结果确定了血清弓形虫血清学模式的血清样品中的弓形虫病的酶联免疫吸附测定（ELISA） 。 ELISA用碱性磷酸酶标记的抗IgG和抗IgM抗体进行。血清滴度表示为仅通过在测试中观察显色确定的血清稀释终点。在古代感染的A组血清（模式II和III）和近期感染的B组血清（模式I）中，IgG-ELISA和IgG-IF滴度之间发现了直接的一致性。在A组血清中IgG-ELISA和血凝滴度之间也发现了类似的协议，对于IgG-IF和血凝滴度也很常见。然而，在B组血清中，尽管使用相同的弓形体提取物来致敏塑料表面和红细胞，但IgG-ELISA效价远高于血凝滴度，其观察方法与IgG-IF效价相似。 B组所有血清中IgM-ELISA均为阳性，滴度高于相应的IgM-IF滴度。 A组血清偶尔会出现低滴度IgM-ELISA阳性结果，有时是由于IgM-抗球蛋白抗体引起的。 ELISA易于执行，似乎可以代替弓形虫IF进行常规检测。

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