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首页> 外文期刊>Infection and immunity >Respiratory Immune Response to Aerosolized Keyhole Limpet Hemocyanin in Primate Lungs
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Respiratory Immune Response to Aerosolized Keyhole Limpet Hemocyanin in Primate Lungs

机译：灵长类动物肺对气雾化匙孔hole血蓝蛋白的呼吸道免疫反应

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Methodology for delivery of antigen to the lower respiratory tract of rhesus monkeys and the recovery of respiratory secretions (RS) by bronchial lavage has been evaluated and has been done on repeated occasions without apparent risk to animals. Rhesus immunoglobulin (Ig)G, IgM, and IgA were identified in RS by their cross-reactivity with human IgG, IgA, and IgM, and relative concentrations of rhesus IgA and IgG could be evaluated in sequential samples of RS and serum (S) by radial immunodiffusion analysis using antiserum specific for human γ and α chains. Administration of keyhole limpet hemocyanin (KLH) by aerosol was compared with intravenous administration of KLH. The aerosol route of administration resulted in IgG, IgM, and IgA antibodies detectable by radio-immunodiffusion in RS but only IgG antibodies in serum. After separate sequences of exposure to KLH delivered to the lung, a secondary type of immune response in the lung appeared as indicated by time of appearance and height of antibody titers. Antibody titers against KLH were estimated by passive hemagglutination of tanned sheep red cells coated with KLH, and anti-KLH titers were comparatively higher in RS of animals immunized by the respiratory route. Treatment of RS and S with 2-mercaptoethanol resulted in reduction of hemagglutination titers of both S and RS, but the degree of reduction varied in different samples and was independent of whether or not the S and RS were collected simultaneously. These results indicate that at least part of the antibody response to aerosolized KLH in rhesus monkeys occurs in the lung and that antibodies may appear in all three immunoglobulin classes in this response.
机译：评估了将抗原递送到恒河猴下呼吸道和通过支气管灌洗恢复呼吸道分泌物（RS）的方法学，并且该方法已多次进行，对动物没有明显危险。通过与人IgG，IgA和IgM的交叉反应，在RS中鉴定了恒河猴免疫球蛋白（Ig）G，IgM和IgA，并且可以在连续的RS和血清样本中评估恒河猴IgA和IgG的相对浓度（S）通过使用对人γ和α链具有特异性的抗血清进行放射免疫扩散分析。将通过气雾剂注射匙孔hole血蓝蛋白（KLH）与静脉注射KLH进行了比较。气雾剂给药途径可通过放射免疫扩散在RS中检测到IgG，IgM和IgA抗体，但血清中仅IgG抗体。在将暴露于KLH的单独暴露序列递送至肺后，肺中出现了第二类免疫应答，如出现时间和抗体滴度的高度所指示。通过对覆有KLH的鞣制绵羊红细胞进行被动血凝而估计了针对KLH的抗体滴度，而在通过呼吸途径免疫的动物的RS中，抗KLH滴度相对较高。用2-巯基乙醇处理RS和S导致S和RS的血凝滴度降低，但降低程度在不同样品中有所不同，并且与是否同时采集S和RS无关。这些结果表明，至少有一部分对猕猴气雾化的KLH的抗体应答发生在肺中，并且该应答中抗体可能出现在所有三种免疫球蛋白类别中。

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