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首页> 外文期刊>Infection and immunity >Fate of 51Cr-labeled lipopolysaccharide in tissue culture cells and livers of normal mice.
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Fate of 51Cr-labeled lipopolysaccharide in tissue culture cells and livers of normal mice.

机译：正常小鼠组织培养细胞和肝脏中51Cr标记的脂多糖的命运。

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Livers of normal mice trapped over 80% of intravenously injected 51Cr-labeled lipopolysaccharide after 1 h. Liver fractionation studies showed that nearly 45% of the labeled endotoxin was associated with cell nuclei, 20% with the mitochondrial-lysosomal fraction, and approximately 30% with the cell sap. Analysis of the distribution of 51Cr-labeled lipopolysaccharide among parenchymal and Kupffer cells showed that over 75% of the in vivo-trapped counts were parenchymal cell associated. Cell populations were approximately 65% parenchymal cells and 35% nonparenchymal cells. Further, six non-reticuloendothelial system tissue culture cell lines were tested for their ability to internalize labeled lipopolysaccharide. In all cells studied, 1 to 4% of the labeled lipopolysaccharide was taken up after 3 h, with greater than 80% of the counts localized in the nuclear fraction. The data show that non-reticuloendothelial system cells can sequester endotoxin both in vivo and in vitro and suggest that parenchymal cells as well as Kupffer cells remove circulating endotoxin from the blood.
机译：1小时后，正常小鼠的肝脏捕获了80％的静脉内注射的51Cr标记的脂多糖。肝分级研究表明，近45％的标记内毒素与细胞核有关，线粒体-溶酶体级分与20％有关，而液汁与约30％有关。对51Cr标记的脂多糖在实质细胞和Kupffer细胞之间的分布的分析表明，体内捕获的计数中超过75％与实质细胞相关。细胞群体为大约65％的实质细胞和35％的非实质细胞。此外，测试了六个非网状内皮系统组织培养细胞系内在化标记的脂多糖的能力。在所有研究的细胞中，3小时后吸收了1-4％的标记脂多糖，其中80％以上的计数位于核级分中。数据表明，非网状内皮系统细胞可以在体内和体外隔离内毒素，这表明实质细胞以及库普弗细胞都可以从血液中去除循环中的内毒素。

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