Chemiluminescence response of phagocytizing human monocytes.

R D Nelson; E L Mills; R L Simmons; P G Quie

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Chemiluminescence response of phagocytizing human monocytes.

机译：吞噬人类单核细胞的化学发光反应。

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Chemiluminescence (CL) occurs as a consequence of phagocytosis of bacteria or inert particles by human polymorphonuclear neutrophils. This phenomenon appears to be related to the production of singlet oxygen and/or electronically excited carbonyl groups which relax with light emission. We report that human monocytes also produce CL after phagocytosis of opsonized bacteria, fungi, or zymosan particles. Monocytes produce approximately one-third of the CL produced by polymorphonuclear neutrophils for all three particles, reflecting possible metabolic differences between cells of these types. Addition of exogenous superoxide dismutase reduced the peak production of CL by 65% for cells of both types, whereas raising the temperature of the cultures from 25 to 37 C approximately doubled the peak CL responses.

机译：化学发光（CL）是由于人类多形核中性粒细胞吞噬细菌或惰性颗粒而导致的。该现象似乎与单线态氧和/或电子激发的羰基的产生有关，所述单线态氧和/或电子激发的羰基随着发光而松弛。我们报告人类单核细胞吞噬调理的细菌，真菌或酵母聚糖颗粒后也产生CL。单核细胞产生的多态核中性粒细胞产生的CL约占全部三个颗粒的三分之一，反映了这些类型细胞之间可能的代谢差异。外源超氧化物歧化酶的添加使两种类型的细胞的CL的峰值产量降低了65％，而将培养物的温度从25℃升高至37℃约使CL的峰值响应加倍。

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《Infection and immunity》 |1976年第1期|共6页
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R D Nelson; E L Mills; R L Simmons; P G Quie;
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中图分类医学免疫学;
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6. Chemiluminescence response of phagocytizing human monocytes. [O] . R D Nelson, E L Mills, R L Simmons, 1976

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Chemiluminescence response of phagocytizing human monocytes.

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