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首页> 外文期刊>Infection and immunity >Adhesion of Escherichia coli to human uroepithelial cells in vitro.
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Adhesion of Escherichia coli to human uroepithelial cells in vitro.

机译：大肠杆菌在体外对人尿道上皮细胞的粘附。

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摘要

Optimal conditions for in vitro adherence of Escherichia coli to uroepithelial cells, previously shown to more efficient for strains causing acute symptomatic than that for strains causing "asymptomatic" urinary tract infections, were investigated. Uroepithelial cells from fresh morning urine of healthy individuals and E. coli bacteria from patients with various forms of urinary tract infeciton were used. Adhesion was found to vary, between individuals and epithelial cell types, with epithelial cell viability, bacterial cultivation medium and growth phase, number of bacteria added to the epithelial cells, and incubation time and temperature. Adhesion was also influenced by variations in pH and osmolarity. Optimal test conditions were obtained with post-log-phase bacterial cultures grown on nutrient broth when 10(8) bacteria were added to 10(5) epithelial cells and incubated for 60 min. Considerable variation was found between experiments done on different days, whereas the variation between duplicates was small. The method described may provide a useful tool in the study of the host-parasite relationship in urinary tract infections.
机译：研究了大肠杆菌在体外粘附于尿道上皮细胞的最佳条件，该条件先前已证明对引起急性症状的菌株比对引起“无症状”尿路感染的菌株更有效。使用来自健康个体新鲜晨尿的尿道上皮细胞和来自患有各种形式尿路感染的患者的大肠杆菌。发现粘附力在个体和上皮细胞类型之间随上皮细胞生存力，细菌培养基和生长期，添加到上皮细胞中的细菌数量以及孵育时间和温度而变化。 pH和渗透压的变化也会影响附着力。当将10（8）细菌添加到10（5）上皮细胞中并孵育60分钟时，在营养肉汤中生长的对数后阶段细菌培养物获得了最佳测试条件。在不同日期进行的实验之间发现差异很大，而重复项之间的差异很小。所描述的方法可以为研究尿路感染中宿主与寄生虫之间的关系提供有用的工具。

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