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首页> 外文期刊>Infection and immunity >Detection of heat-labile enterotoxin-like activity in stools of patients with cholera and Escherichia coli diarrhea.
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Detection of heat-labile enterotoxin-like activity in stools of patients with cholera and Escherichia coli diarrhea.

机译:霍乱和大肠杆菌性腹泻患者粪便中不耐热肠毒素样活性的检测。

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The Y1 adrenal cell tissue culture assay was used to detect heat-labile enterotoxin-like activity in the stools of 14 of 74 patients with diarrhea. A positive effect of the stool on the adrenal cells was heat-labile and neutralized by cholera antitoxin. Enterotoxin-like activity was detected in the stools of 10 of 30 patients with cholera and in those of 2 of 4 from whom heat-labile Escherichia coli were isolated. None of the stools from nine individuals with Vibrio parahaemolyticus, Salmonella, or Shigella infections were positive. Two of 31 individuals from whom no pathogens were isolated had detectable toxin-like activity in their stools. The Y1 adrenal cell assay provides a rapid method of diagnosing heat-labile enterotoxigenic diarrhea and could be an adjunct in epidemiological studies of gastroenteritis.
机译:使用Y1肾上腺细胞组织培养法检测74例腹泻患者中14例的粪便中热不稳定性肠毒素样活性。粪便对肾上腺细胞的积极作用是热不稳定的,并被霍乱抗毒素中和。在30例霍乱患者中有10例粪便中检出了肠毒素样活性,在分离出热不稳定大肠杆菌的4例中有2例中检出了肠毒素样活性。 9例副溶血性弧菌,沙门氏菌或志贺氏菌感染患者的粪便均无阳性。在未分离出病原体的31个人中,有2个人的粪便中具有可检测到的毒素样活性。 Y1肾上腺细胞测定法提供了一种诊断不耐热肠毒素的腹泻的快速方法,并且可能是胃肠炎流行病学研究的辅助手段。

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