With a purified preparation of pneumococcal hemolysin, a sigmoid relationship was found to exist between lysin concentration and hemolytic activity. Hemolysis was inhibited by a high ratio of erythrocytes to lysin, suggesting a multi-hit mechanism of action. Reaction rate decreased rapidly with time, possibly due to competition between ghosts and unlysed erythrocytes for fixation of lysin. The effects of pH and various agents on the processes of lysin adsorption and hemoglobin release were determined. The pneumococcal preparation did not possess nicotinamide adenine dinucleotide glycohydrolase activity.
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机译：用肺炎球菌溶血素的纯化制剂，发现溶血素浓度与溶血活性之间存在乙状关系。溶血被高比例的红细胞与溶血素抑制，表明其作用机制多种多样。反应速率随时间迅速降低，可能是由于鬼影和未溶解的红细胞之间对溶血素的固定所进行的竞争。确定了 p H和各种试剂对溶菌素吸附和血红蛋白释放过程的影响。肺炎球菌制剂不具有烟酰胺腺嘌呤二核苷酸糖水解酶活性。
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