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Isolation by Electrofocusing of Two Lymphocyte Mitogens Produced by Staphylococcus aureus

机译：通过电聚焦分离金黄色葡萄球菌产生的两种淋巴细胞有丝分裂原

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Staphylococcus aureus strain DA352, grown in a diffusate of Todd-Hewitt broth, produced two extracellular nondialyzable lymphocyte mitogens having isoelectric points of 5.5 to 5.7 and 8.6 to 9.0. The mitogens were separable from one another by isoelectric focusing and could be isolated free of detectable amounts of other staphylococcal products by ethanol precipitation followed by isoelectric focusing. Dose-response curves with both mitogens showed a maximum per cent transformation in the range (90%+) obtained with phytohemagglutinin (PHA), a decrease of transformation with excess mitogen, and, with decreasing concentrations, a slope somewhat less steep than that obtained with PHA. Incubation with undigested or pepsin-digested pooled human gamma globulin enhanced the activity of the basic mitogen.
机译：在Todd-Hewitt肉汤的扩散液中生长的金黄色葡萄球菌菌株DA352产生了两种等电点分别为5.5至5.7和8.6至9.0的细胞外不可透析淋巴细胞有丝分裂原。有丝分裂原可以通过等电聚焦彼此分离，并且可以通过乙醇沉淀然后等电聚焦分离出可检测量的其他葡萄球菌产物。两种促细胞分裂剂的剂量反应曲线显示，在使用植物血凝素（PHA）获得的范围内，转化率最大（90％+），随着促细胞分裂剂的过量而降低，并且随着浓度的降低，斜率比获得的斜率略低与PHA。与未消化或胃蛋白酶消化的合并的人γ球蛋白一起孵育可增强碱性促分裂原的活性。

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