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首页> 外文期刊>Infection and immunity >Suppression of the Intracellular Growth of Shigella flexneri in Cell Cultures by Interferon Preparations and Polyinosinic-Polycytidylic Acid
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Suppression of the Intracellular Growth of Shigella flexneri in Cell Cultures by Interferon Preparations and Polyinosinic-Polycytidylic Acid

机译：干扰素制剂和聚肌苷酸-聚胞苷酸抑制弗氏志贺氏菌在细胞培养中的细胞内生长

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A strain of Shigella flexneri type 2a was found to multiply intracellularly in cultures of the diploid human cell strain FS-1 and in secondary rabbit kidney cells. When inoculated cultures were stained and observed under the light microscope, it appeared that the cytoplasm of infected cells became gradually filled with bacteria. Various preparations of human and rabbit interferon were found to suppress the intracellular bacterial growth in homologous cells. Polyinosinic-polycytidylic acid (poly I·poly C) had a similar inhibitory effect. Rabbit interferon preparations did not cause a significant suppression in human cells. Suppression of the bacterial growth could be demonstrated in two ways: (i) by showing that treatment with either homologous interferon or poly I·poly C reduced the proportion of infected cells determined by counting the total number of cells and the number of cells with 10 or more bacteria in several microscopic fields selected at random, or (ii) by showing that a suppression in 3H-uridine incorporation by bacteria occurs in infected cultures treated with actinomycin D after incubation with interferon or poly I·poly C. (Uridine incorporation by the bacterium is insensitive to actinomycin D.) Treatment of cells with actinomycin D before incubation with interferon prevented the development of cellular resistance to bacterial infection. Interferon preparations did not have an inhibitory effect on the extracellular growth of S. flexneri in a broth culture. These findings show that the range of activity of the interferon system apparently extends to intracellularly growing bacteria.
机译：在二倍体人类细胞株FS-1和次生兔肾细胞中，发现了一种弗氏志贺氏菌 2a型在细胞内繁殖。当将接种的培养物染色并在光学显微镜下观察时，似乎感染细胞的细胞质逐渐被细菌填充。发现人和兔干扰素的各种制剂可抑制同源细胞中细胞内细菌的生长。聚肌苷酸-聚胞苷酸（poly I·poly C）具有相似的抑制作用。兔干扰素制剂未对人体细胞产生明显抑制作用。抑制细菌生长的方法有两种：（i）通过显示用同源干扰素或poly I·poly C进行的处理可以减少感染细胞的比例，该比例是通过计算细胞总数和10个细胞数来确定的随机选择的几个显微镜视野中的一个或多个细菌，或（ii）通过显示在与干扰素或聚I孵育后用放线菌素D处理的感染培养物中，细菌抑制了 3 H-尿苷掺入·多聚丙酸（细菌掺入的尿苷对放线菌素D不敏感）。在与干扰素孵育前用放线菌素D处理细胞可防止细胞产生对细菌感染的抗性。干扰素制剂对 S的细胞外生长没有抑制作用。肉汤培养中的弗氏菌。这些发现表明，干扰素系统的活性范围显然扩展到细胞内生长的细菌。

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