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首页> 外文期刊>Infection and immunity >Cytological Localization of Dengue-2 Antigens: an Immunological Study with Ultrastructural Correlation
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Cytological Localization of Dengue-2 Antigens: an Immunological Study with Ultrastructural Correlation

机译：登革2抗原的细胞学定位：与超微结构相关的免疫学研究。

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Immunofluorescence and electron microscopy were used to study the development and cytological distribution of dengue-2 virion and nonvirion antigens in monkey kidney cells. The type and combination of fixatives were found to affect the intensity of fluorescence. Paraformaldehyde fixation alone resulted in a low level of fluorescence, but an additional fixation step with ethanol or acetone resulted in maximum staining. No fluorescence was obtained after fixation with glutaraldehyde alone or in combination with other solvents. Methanol caused selective ablation of fluorescence by antibody to purified virions. Dengue-specific fluorescence that was most intense in the perinuclear area radiated in a granular pattern of decreasing intensity into the cytoplasm. The perinuclear fluorescence was associated with nonvirion antigens and the cytoplasmic fluorescence was associated with virion antigens. Electron micrographs of infected cells revealed vesicular bodies with reticular electron-dense centers, clusters of structurally complete virions deep within the endoplasmic reticulum, and single virus particles in vacuoles near the periphery of the cell.
机译：免疫荧光和电子显微镜用于研究猴肾细胞中登革2病毒粒子和非病毒粒子抗原的发育和细胞学分布。发现固定剂的类型和组合会影响荧光强度。单独使用低聚甲醛固定会导致荧光水平降低，但是使用乙醇或丙酮进行额外的固定步骤会导致最大程度的染色。单独使用戊二醛或与其他溶剂混合固定后，未获得荧光。甲醇导致纯化的病毒粒子抗体选择性消融荧光。在核周区域中最强烈的登革热特异性荧光以强度降低的颗粒状辐射到细胞质中。核周荧光与非病毒粒子抗原有关，而胞质荧光与病毒粒子抗原有关。被感染细胞的电子显微照片显示出具有网状电子致密中心的囊泡体，在内质网深处结构完整的病毒体簇，并且在细胞外围附近的液泡中有单个病毒颗粒。

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