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首页> 外文期刊>Infection and immunity >Cellular location of alpha-hemolysin in Staphylococcus aureus.
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Cellular location of alpha-hemolysin in Staphylococcus aureus.

机译:α-溶血素在金黄色葡萄球菌中的细胞位置。

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Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.
机译:原生质体分级分离技术用于制备三种金黄色葡萄球菌菌株的亚细胞级分,以分析α-溶血素的活性。超过90%的细胞溶血活性位于周质中,在细胞质中仅检测到痕量,在质膜或介体中均未发现。该细胞部分约占培养物总溶血活性的4-10%。红细胞裂解光谱和Sephadex G-100过滤研究表明,细胞溶血素与细胞外形式非常相似。

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