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首页> 外文期刊>Infection and immunity >Delayed hypersensitivity responses of experimental animals to histoplasmin from the yeast and mycelial phases of Histoplasma capsulatum.
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Delayed hypersensitivity responses of experimental animals to histoplasmin from the yeast and mycelial phases of Histoplasma capsulatum.

机译：延缓了实验动物对荚膜组织胞浆菌的酵母和菌丝体组织胞浆蛋白的超敏反应。

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摘要

Controlled yeast lysate (CYL) and controlled mycelial lysate (CML) histoplasmins were produced from Histoplasma capsulatum grown in a nutritionally lean, chemically defined medium. The lysates were assayed for skin-test activity in guinea pigs sensitized by infection with the homologous organism. In some studies, nonliving vaccine preparations were employed also. Inter-lot biological variation was minimal, and 20 lots of the CYL reagent elicited strong dermal reactions with high specificity. Further, CYL reagents were nonreactive in guinea pigs infected with Coccidioides immitis, whereas the commercial Food and Drug Administration preparations cross-reacted to some degree. The CML histoplasmins were generally less reactive than the CYL preparations and exhibited somewhat more inter-lot variation in sensitivity and specificity. No correlation between potency and protein:polysaccharide ratios were observed with either reagent. An intradermal test with the CYL reagent did not induce significant changes in the complement-fixing titer of sensitized guinea pigs. Such changes in sensitized animals were elicited by a skin test with commercial histoplasmin.
机译：受控的酵母裂解物（CYL）和受控的菌丝体裂解物（CML）的组织纤溶酶是从在营养贫化，化学成分确定的培养基中生长的荚膜组织胞浆产生的。在通过感染同源生物而致敏的豚鼠中，测定了裂解物的皮肤测试活性。在一些研究中，还使用了无生命的疫苗制剂。批次间的生物学差异极小，并且20批CYL试剂引发了高特异性的强烈皮肤反应。此外，CYL试剂在感染球虫科动物的豚鼠中没有反应性，而商业食品和药物管理局的制剂则在一定程度上发生交叉反应。 CML组织浆蛋白的反应性通常不及CYL制剂，并且在批间敏感性和特异性方面表现出更大的差异。两种试剂均未观察到效价与蛋白质：多糖比例之间的相关性。用CYL试剂进行的皮内试验未引起致敏豚鼠补体固定效价的显着变化。致敏动物的这种变化是通过商业化组织浆蛋白的皮肤试验引起的。

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