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首页> 外文期刊>Infection and immunity >Interaction of polymorphonuclear leukocytes with smooth and rough strains of Brucella abortus.
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Interaction of polymorphonuclear leukocytes with smooth and rough strains of Brucella abortus.

机译：多形核白细胞与流产布鲁氏菌平滑和粗糙菌株的相互作用。

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The bactericidal activity of guinea pig and human polymorphonuclear leukocytes (PMNs) against a smooth-intermediate strain (45/0) and a rough strain (45/20) of Brucella abortus has been examined. After incubation for 120 min, guinea pig PMNs incubated with either the smooth strain 45/0 or the rough strain 45/20 exhibited no bactericidal activity against the former and caused only a 34% decrease in viability of the latter. Human PMNs were more bactericidal than guinea pig PMNs to both strains; however, the killing of strain 45/20 by human PMNs was less than that observed in control experiments with S. aureus strain 502A. Both strains of B. abortus readily associated with guinea pig and human PMNs, and the bacteria were apparently ingested without stimulation of the hexose monophosphate pathway. Lysates (10 micrograms/ml, pH 5.5), prepared from guinea pig or human granules, were not particularly toxic to either strain unless supplemented with H2O2 and a halide (I- or Cl-). An oxygen-dependent killing system appeared to be lethal against both strains of B. abortus, with I- being more active than Cl- in the presence of H2O2 and granule lysate. The data suggest that degranulation after ingestion of Brucella by phagocytes does not occur due to the lack of a proper stimulus or possibly the baccilli actively inhibit the degranulation process thereby protecting the microbe from killing systems normally effective against extracellular parasites.
机译：研究了豚鼠和人多形核白细胞（PMN）对流产布鲁氏菌的平滑中间菌株（45/0）和粗菌株（45/20）的杀菌活性。温育120分钟后，用光滑菌株45/0或粗糙菌株45/20温育的豚鼠PMN对前者没有杀菌活性，而后者的存活率仅降低了34％。在两种菌株中，人PMN比豚鼠PMN更具杀菌性。然而，人PMN对45/20菌株的杀灭作用低于金黄色葡萄球菌502A对照实验所观察到的杀伤力。流产双歧杆菌的两种菌株都容易与豚鼠和人PMN相关，并且显然在不刺激己糖单磷酸途径的情况下摄入了细菌。从豚鼠或人颗粒制备的裂解物（10微克/毫升，pH 5.5）对任何一种菌株均无特殊毒性，除非补充了H2O2和卤化物（I-或Cl-）。氧依赖性杀伤系统似乎对流产双歧杆菌均具有致命性，在H2O2和颗粒裂解物的存在下，I-的活性高于Cl-。数据表明，由于缺乏适当的刺激，吞噬细胞摄取布鲁氏菌后不会发生脱粒，或者杆菌可能会主动抑制脱粒过程，从而保护微生物免受杀死通常对细胞外寄生虫有效的系统。

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