Some Physical Properties of K Antigens of Escherichia coli Related to Their Biological Activity

C. J. Howard; A. A. Glynn

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Some Physical Properties of K Antigens of Escherichia coli Related to Their Biological Activity

机译：大肠杆菌K抗原的某些物理性质与其生物学活性相关

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The ability of K antigens from Escherichia coli to inhibit the agglutination of sheep red cells by rabbit antibody has been taken as a measure of the ability of such antigens to render strains of E. coli O-inagglutinable, resistant to complement and to phagocytosis, and to increase their virulence for mice. Analysis of K antigens by chromatography on Sepharose and diethylaminoethyl cellulose columns and by immunoelectrophoresis suggested that agglutination-inhibiting activity was directly related to molecular size rather than charge. The effect of alkali treatment showed inhibitory activity to be independent of K-specific antigenic determinants and confirmed its relation to molecular weight.

机译：来自大肠杆菌的K抗原抑制兔抗体对绵羊红细胞凝集的能力已被用作衡量此类抗原产生大肠杆菌的能力的一种量度。大肠杆菌O型可凝集，对补体和吞噬作用有抵抗力，并能增加小鼠的毒力。通过琼脂糖凝胶和二乙基氨基乙基纤维素柱上的色谱分析以及通过免疫电泳分析K抗原，表明凝集抑制活性与分子大小直接相关，而不是电荷。碱处理的效果显示抑制活性与K特异性抗原决定簇无关，并证实了其与分子量的关系。

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《Infection and immunity》 |1971年第1期|共6页
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C. J. Howard; A. A. Glynn;
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1. Escherichia coli heat-labile toxin subunit B fusions with Streptococcus sobrinus antigens expressed by Salmonella typhimurium oral vaccine strains: importance of the linker for antigenicity and biological activities of the hybrid proteins. [J] . E K Jagusztyn-Krynicka, J E Clark-Curtiss, R Curtiss Infection and immunity . 1993,第3期

机译：大肠杆菌热不稳定毒素亚基B与鼠伤寒沙门氏菌口服疫苗株表达的链球菌链球菌抗原融合：接头对于杂合蛋白的抗原性和生物学活性的重要性。
2. Escherichia coli heat-labile toxin subunit B fusions with Streptococcus sobrinus antigens expressed by Salmonella typhimurium oral vaccine strains: importance of the linker for antigenicity and biological activities of the hybrid proteins. [J] . E K Jagusztyn-Krynicka, J E Clark-Curtiss, R Curtiss Infection and immunity . 1993,第3期

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3. An Escherichia Coli Antigen Cross-Reactive with the Capsular Polysaccharide of Haemophilus Influenzae Type b: Occurrence Among Known Serotypes, and Immunochemical and Biologic Properties of E. Coli Antisera Toward H. Influenzae Type b [J] . J. C. Parke, John B. Robbins, John K. Whisnant, The journal of immunology . 1972,第6期

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5. Physical-chemical characterization and biological activity of lipopolysaccharides released from Escherichia coli by the action of normal human serum [D] . Tesh, Vernon Lewis. 1989

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6. Some Physical Properties of K Antigens of Escherichia coli Related to Their Biological Activity [O] . C. J. Howard, A. A. Glynn 2020

机译：大肠杆菌K抗原的某些物理性质与其生物学活性相关
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8. Genotypic Characterization of Egypt Enterotoxigenic Escherichia coli Isolates Expressing Coli Surface Antigen 6. [R] . A. Mansour A. M. El-Gendy A. W. Armstrong H. I. Shaheen M. R. Monteville 2013

机译：表达大肠杆菌表面抗原的埃及肠产毒性大肠杆菌分离株的基因型特征。

Some Physical Properties of K Antigens of Escherichia coli Related to Their Biological Activity

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