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首页> 外文期刊>Infection and immunity >Mechanisms of Immunity in Typhus Infections II. Multiplication of Typhus Rickettsiae in Human Macrophage Cell Cultures in the Nonimmune System: Influence of Virulence of Rickettsial Strains and of Chloramphenicol
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Mechanisms of Immunity in Typhus Infections II. Multiplication of Typhus Rickettsiae in Human Macrophage Cell Cultures in the Nonimmune System: Influence of Virulence of Rickettsial Strains and of Chloramphenicol

机译：斑疹伤寒感染的免疫机制II。斑疹伤寒立克次体在非免疫系统中的人类巨噬细胞培养中的繁殖：立克次体菌株和氯霉素的毒力的影响

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Monocytes from the peripheral blood of nonimmune human subjects transformed in cell culture into macrophages with increased phagocytic capacity for killed typhus rickettsiae. When such cells were exposed to living virulent Rickettsia mooseri (Wilmington strain) or R. prowazeki (Breinl strain), or to the attenuated E strain of R. prowazeki, in the presence of medium containing normal human serum, all three strains readily entered the macrophage, but the subsequent fate varied according to strain and its virulence. Thus, R. mooseri grew readily to attain very high intracellular populations which eventually destroyed the macrophage in 3 to 5 days and escaped to infect other cells. Virulent R. prowazeki also grew at about the same rate for the first 2 to 3 days but then often abruptly ceased to multiply. Circumstantial evidence suggests a toxic effect on host cells by smaller numbers of R. prowazeki organisms than with R. mooseri. The attenuated E strain of R. prowazeki failed to grow in most cells and eventually disappeared, but did grow to substantial numbers in the very rare cell in some cultures, suggesting the presence of a few cells which may not be typical macrophages. The growth of R. mooseri in the macrophage cytoplasm was inhibited by chloramphenicol in the culture medium. When the drug was removed after 3 days, growth began after a lag period and assumed a normal rate.
机译：非免疫人类受试者外周血中的单核细胞在细胞培养中转化为巨噬细胞，吞噬能力增强了对立克次体的杀死。当此类细胞暴露于有生命力的强力立克次氏酵母（Wilmington菌株）或 R时。 prowazeki （Breinl株），或减毒的 R E株。在含有正常人血清的培养基中，所有三种菌株都容易进入巨噬细胞，但随后的命运因菌株及其毒力而异。因此， R。穆斯里（Mooseri）易于生长以达到很高的细胞内种群，最终在3至5天之内破坏了巨噬细胞并逃脱了，以感染其他细胞。剧毒 R。前2到3天，prowazeki 的生长速度也差不多，但随后突然停止繁殖。间接证据表明较少数量的 R对宿主细胞有毒性作用。 prowazeki 生物体比 R。 R的减毒E株。 prowazeki 不能在大多数细胞中生长，最终消失，但在某些培养物中的非常罕见的细胞中却确实生长了很多，这表明存在一些可能不是典型巨噬细胞的细胞。 R的增长。培养基中的氯霉素可抑制巨噬细胞胞质中的Mooseri 。 3天后撤除药物时，经过一段时间的滞后后开始生长，并假定速度正常。

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