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首页> 外文期刊>Infection and immunity >Immunochemical analysis of serum-related proteins in the respiratory tract secretions of normal mice.
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Immunochemical analysis of serum-related proteins in the respiratory tract secretions of normal mice.

机译：正常小鼠呼吸道分泌物中血清相关蛋白的免疫化学分析。

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Normal mouse lung lavage fluid was analyzed for its content of serum-related proteins by the methods of gel filtration, immunoelectrophoresis, and double immunodiffusion. Lavage samples were collected from exsanguinated mice using three repeated infusions of 0.9% sodium chloride solution. Cells were removed, and the lavage fluid was concentrated. Approximately 0.23 mg of protein per mouse was present in the concentrated solution. Albumin, transferrin, and immunoglobulin G (IgG) were among the proteins in greatest concentration. Albumin, IgG1, IgG2, IgA, and IgM were quantitated by single radial diffusion. Although immunoglobulins could be synthesized locally, albumin and transferrin were assumed to come from plasma. The albumin content was used to estimate the amount of transudated plasma as 0.0027 ml per mouse. The IgA levels were low with an IgA/IgG1 ratio of 0.58 and an IgA/IgG2 ratio of 0.43. IgM was not detected. Goat anti-lung lavage serum revealed the presence of additional lung-related proteins.
机译：通过凝胶过滤，免疫电泳和双重免疫扩散的方法分析了正常小鼠肺灌洗液中血清相关蛋白的含量。使用3次重复输注0.9％氯化钠溶液从放血小鼠中收集洗胃样品。除去细胞，并浓缩灌洗液。每只小鼠的浓缩溶液中存在约0.23 mg的蛋白质。白蛋白，转铁蛋白和免疫球蛋白G（IgG）浓度最高。通过单次径向扩散对白蛋白，IgG1，IgG2，IgA和IgM进行​​定量。尽管免疫球蛋白可以在本地合成，但白蛋白和转铁蛋白被认为来自血浆。用白蛋白含量估计渗出血浆的量为每只小鼠0.0027ml。 IgA水平较低，IgA / IgG1比为0.58，IgA / IgG2比为0.43。未检测到IgM。山羊抗肺灌洗血清显示存在其他与肺相关的蛋白质。

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