• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization of an antibody directed against a surface component of normal and pleomorphic cells of Streptococcus sanguis.
【24h】

Characterization of an antibody directed against a surface component of normal and pleomorphic cells of Streptococcus sanguis.

机译：针对血链球菌正常和多形细胞表面成分的抗体的表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Whole cells of Streptococcus sanguis were utilized as an immunoadsorbent to purify large quantities of an antibody (S1) directed against a cell surface component. The S-1 antibody was isolated from antisera to normal (N) and pleomorphic (O) cells by a similar adsorption-elution procedure. The S-1 antibody isolated from antisera to N cells reacted in gel diffusion in identify with the S-1 antibody to O cells, indicating that the antigen which binds S-1 antibody (Ag-1) may not be radically altered when cells become pleomorphic. The S-1 antibodies directed against both N and O cells had restricted heterogeneity, indicating that for both types of cell Ag-1 may have a simple repeating structure. However, N cells were agglutinated to a greater extent by S-1 antibody than O cells. In addition the distribution of the bound S-1 antibody became altered as the cells became pleomorphic. Utilizing the technique of indirect immunofluorescence we observed that the S-1 antibody was distributed evenly on the surface of N cells. As the cells became pleomorphic, the antibody appeared to bind preferentially at the cell poles (capping). Later, as the cells became more grossly deformed, additional bands of immunofluorescence appeared to bisect the cells. Electron microscopic analysis indicated that the bound antibody was not associated with septal notches. The results suggest that the arrangement rather than the immunological properties of Ag-1 became altered as cells became pleomorphic.
机译：血链球菌的整个细胞被用作免疫吸附剂，以纯化针对细胞表面成分的大量抗体（S1）。通过类似的吸附-洗脱程序，从抗血清中分离出S-1抗体至正常（N）和多形性（O）细胞。从抗血清分离到N细胞的S-1抗体在凝胶扩散中起反应，从而与针对O细胞的S-1抗体相鉴别，这表明与S-1抗体（Ag-1）结合的抗原可能不会在细胞变成多态的。针对N细胞和O细胞的S-1抗体具有有限的异质性，这表明对于两种类型的细胞，Ag-1可能都具有简单的重复结构。然而，与O细胞相比，S-1抗体对N细胞的凝集程度更大。另外，随着细胞变得多形性，结合的S-1抗体的分布也改变了。利用间接免疫荧光技术，我们观察到S-1抗体均匀分布在N细胞表面。随着细胞变得多形性，抗体似乎优先在细胞极结合（加帽）。后来，随着细胞变得更加严重的变形，免疫荧光的其他条带似乎将细胞一分为二。电子显微镜分析表明结合的抗体与间隔切口不相关。结果表明，随着细胞变得多形性，Ag-1的排列方式而不是免疫特性发生了改变。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号