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首页> 外文期刊>Infection and immunity >Lymphocyte response to T-cell mitogen during experimental gingivitis in humans.
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Lymphocyte response to T-cell mitogen during experimental gingivitis in humans.

机译：在人类实验性牙龈炎中，淋巴细胞对T细胞有丝分裂原的反应。

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This study was conducted to evaluate the dose response relationships of peripheral blood lymphocytes (PBL) by stimulation of phytohemagglutinin (PHA) during the onset of oral inflammation. Eleven dental students underwent a 3-week experimental gingivitis program (L?e et al., 1965). At time zero, weeks 1, 2, and 3, and after 1 week of reinstituted oral hygiene (week 4), the plaque accumulations were evaluated, the degree of gingival inflammation was assessed, and a blood sample was taken. Quadruplicate microcultures each containing 2 x 10(5) PBL in 0.2 ml of tissue culture medium 199 and 10% fetal calf serum were stimulated with five concentrations of PHA (10 to 0.5 mug/ml) and incubated for 78 h at 37 C in 5% CO2. [3H]thymidine was added to each culture for the final 8 h. The cultures were then harvested and counted by liquid scintillation, and stimulation indexes (SI) were determined. At time zero the maximum PBL response occurred at a PHA concentration of 5 mug/ml (SI = 100). During weeks 1, 2, and 3 the location of the maximum PBL response shifted to a lower PHA concentration (1.0 mug/ml) and increased to over SI =400. The phenomenon of shifting peak PHA responses to lower PHA concentrations could be observed after only 1 week of developing gingival inflammation. The PBL response returned to pre-experimental values after 1 week of reinstituted oral hygiene, which resolved the oral inflammation. The findings show that a dose response relationship exists between PHA concentrations and the PBL response. If these dose response changes seen during developing gingival inflammation are ignored, either a decrease, increase, or no change in PBL response can be shown depending upon the PHA concentration evaluated. Owing to the dose-dependent nature of this PBL response, it is advisable to routinely use dose response curves in order to properly evaluate the full responsiveness of PBL to mitogenic substances.
机译：进行此项研究以评估口腔炎症发作期间通过刺激植物血凝素（PHA）引起的外周血淋巴细胞（PBL）的剂量反应关系。十一名牙科学生接受了为期3周的实验性牙龈炎计划（L？e等，1965）。在零时，第1、2和3周以及恢复口腔卫生1周后（第4周），评估了斑块堆积，评估了牙龈炎症的程度，并采集了血液样本。用五种浓度的PHA（10到0.5杯/毫升）刺激在0.2 ml组织培养基199和10％胎牛血清中分别含有2 x 10（5）PBL的一式四份微量培养物，在37°C下于5中孵育78小时％CO2。在最后8小时内将[3 H]胸苷加入每种培养物中。然后收获培养物并通过液体闪烁计数，并确定刺激指数（SI）。在零时间，PHA浓度为5杯/毫升（SI = 100）时，出现最大的PBL反应。在第1、2和3周，最大PBL反应的位置转移到较低的PHA浓度（1.0杯/毫升），并增加到超过SI = 400。在牙龈发炎仅1周后，就可以观察到峰值PHA响应向较低PHA浓度转移的现象。在恢复口腔卫生1周后，PBL反应恢复到实验前的水平，从而解决了口腔炎症。研究结果表明，PHA浓度与PBL反应之间存在剂量反应关系。如果忽略了在牙龈发炎过程中看到的这些剂量反应变化，则可以根据评估的PHA浓度显示PBL反应的降低，增加或没有变化。由于这种PBL反应的剂量依赖性，建议常规使用剂量反应曲线，以正确评估PBL对促有丝分裂物质的完全反应性。

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