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首页> 外文期刊>Infection and immunity >Effect of in vitro adaptation of Marek's disease virus on pock induction on the chorioallantoic membrane of embryonated chicken eggs.
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Effect of in vitro adaptation of Marek's disease virus on pock induction on the chorioallantoic membrane of embryonated chicken eggs.

机译：马立克氏病病毒体外适应对鸡胚绒毛膜绒毛诱导痘疱的影响。

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摘要

Cell-associated preparations of several isolates of Marek's disease virus produced more pocks on the chorioallantoic membrane of embryonated chicken eggs than plaques in duck embryo fibroblasts, thus indicating that lesion response in eggs was more sensitive than cytopathic response in duck embryo fibroblasts for assaying low-passage Marek's disease virus. Adaptation of the virus to cell cultures by serial passages, however, substantially reduced its pock response so that the titer ratio (plaque-forming units in duck embryo fibroblasts/pock-forming units in eggs) of cell culture-adapted Marek's disease virus was 1 or higher. The decreased pock response could not be attributed to selection of preexisting virus variant(s) with low affinity for chorioallantoic membrane because cloned Marek's disease virus had a good pock response at low cell culture passage levels, but this response decreased as the virus was attenuated by serial cell culture passage.
机译：几种分离的马立克氏病病毒的细胞相关制剂在雏鸡胚蛋的绒毛膜上产生的produced比鸭胚成纤维细胞中的噬菌斑要多，因此表明，对于鸭胚成纤维细胞而言，蛋鸡的病灶反应比细胞病变反应更敏感，从而可检测出低马立克氏病病毒。然而，通过连续传代使病毒适应细胞培养物，大大降低了其痘疱反应，因此适应细胞培养的马立克氏病病毒的效价比（鸭胚成纤维细胞中的噬斑形成单位/卵中的痘疱形成单位）的效价比为1或更高。鸡痘反应降低不能归因于对绒毛膜尿酸膜亲和力低的先前存在的病毒变种的选择，因为克隆的马立克氏病病毒在低细胞培养传代水平下具有良好的鸡痘反应，但是随着病毒的减毒，这种反应降低了。连续细胞培养传代。

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