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首页> 外文期刊>Infection and immunity >In vivo studies on the cellular source of migration inhibitory factor in mice with delayed hypersensitivity.
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In vivo studies on the cellular source of migration inhibitory factor in mice with delayed hypersensitivity.

机译:迟发性超敏反应小鼠体内细胞迁移抑制因子来源的研究。

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Mice sensitized intravenously with 300 microgram of BCG cell walls in Drakeol-Tween 80 and challenged intravenously 3 weeks later with 50 mg of old tuberculin released migration inhibitory factor (MIF) into the circulating blood in quantities that could be detected in serum dilutions of 1:64 to 1:128. When thymus-derived lymphocytes (T-cells) were absent at the time of sensitization, as in neonatally thymectomized mice or in athymic nude mice, detectable amounts of MIF were not formed. Sensitized mice treated with either anti-theta serum or anti-bone marrow-derived lymphocyte (B-cell) serum before intravenous challenge with old tuberculin released reduced amounts of MIF into the circulation. Mice lethally irradiated, reconstituted with B-cells, sensitized with BCG cell walls, and then challenged intravenously 3 weeks later with old tuberculin did not release MIF into the circulation. When T-cells were injected at least 10 days before challenge, however, MIF appeared.
机译:小鼠在Drakeol-Tween 80中用300微克BCG细胞壁静脉内致敏,并在3周后用50 mg旧结核菌素静脉内攻击,向循环血液中释放迁移抑制因子(MIF),其数量可在1的血清稀释液中检测到: 64至1:128。当在致敏时不存在胸腺来源的淋巴细胞(T细胞)时,如在新生儿经胸腺切除的小鼠或无胸腺的裸鼠中,未形成可检测量的MIF。用抗theta血清或抗骨髓源性淋巴细胞(B细胞)血清处理过的致敏小鼠,在用旧结核菌素静脉内攻击之前,将减少的MIF释放到循环系统中。致死性照射小鼠,用B细胞重建,用BCG细胞壁敏化,然后在3周后用旧的结核菌素静脉内攻击,但未将MIF释放到循环中。但是,在攻击前至少10天注射T细胞时,会出现MIF。

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