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首页> 外文期刊>Infection and immunity >Differential chemical protection of mammalian cells from the exotoxins of Corynebacterium diphtheriae and Pseudomonas aeruginosa.
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Differential chemical protection of mammalian cells from the exotoxins of Corynebacterium diphtheriae and Pseudomonas aeruginosa.

机译：区别于白喉棒状杆菌和铜绿假单胞菌外毒素的哺乳动物细胞的差异化学保护。

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Many drugs or chemicals had markedly different effects on the cytotoxicity induced by Pseudomonas aeruginosa exotoxin A (PE) or Corynebacterium diphtheriae exotoxin (DE). The glycolytic inhibitor NaF protected cells from DE but potentiated the cytotoxicity of PE. Another energy inhibitor, salicylic acid, also protected cells from DE but had no effect with PE. Colchicine and colcemid did not affect the cytotoxicity of either toxin. Cytochalasin B exhibited a modest protection from DE but no effect with PE. Ouabain, a specific inhibitor of the Na+, K+-dependent adenosine 5'-triphosphatase (ATPase), did not affect the cytotoxicity of either toxin. Ruthenium red, a specific inhibitor of the Ca2+, Mg2+,-dependent ATPase, conferred marked protection from DE-induced cytotoxicity but did not affect PE-induced cytotoxicity. A number of local anesthetics were tested, and they too presented differential results with PE and DE. Most chemicals that affected toxin-induced cytotoxicity had little or no influence on the in vitro adenosine 5'-diphosphate-ribosylation catalyzed by either toxin. This work presents further evidence that PE and DE have different mechanisms of intoxication and suggests that these differences lie in the attachment or internalization stages of intoxication.
机译：许多药物或化学物质对铜绿假单胞菌外毒素A（PE）或白喉棒状杆菌外毒素（DE）诱导的细胞毒性具有明显不同的影响。糖酵解抑制剂NaF保护细胞免受DE侵害，但增强PE的细胞毒性。另一种能量抑制剂水杨酸也可保护细胞免受DE损害，但对PE无作用。秋水仙碱和秋水仙胶不影响任何一种毒素的细胞毒性。细胞松弛素B对DE表现出适度的保护作用，但对PE无作用。哇巴因是Na +，K +依赖性腺苷5'-三磷酸酶（ATPase）的特异性抑制剂，不影响任何一种毒素的细胞毒性。钌红，一种特定的Ca2 +，Mg2 +依赖性ATPase抑制剂，可显着保护DE诱导的细胞毒性，但不影响PE诱导的细胞毒性。测试了许多局部麻醉剂，它们也与PE和DE表现出不同的结果。影响毒素诱导的细胞毒性的大多数化学物质对两种毒素催化的体外腺苷5'-二磷酸核糖基化影响很小或没有影响。这项工作提供了进一步的证据，证明PE和DE具有不同的中毒机理，并表明这些差异在于中毒的依恋或内在化阶段。

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