Effect of Alkali on the Immunological Reactivity of Lipopolysaccharide from Salmonella typhimurium

Ivan ?i?nár; J. W. Shands

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Effect of Alkali on the Immunological Reactivity of Lipopolysaccharide from Salmonella typhimurium

机译：碱对鼠伤寒沙门氏菌脂多糖免疫活性的影响

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The effect of mild alkaline hydrolysis on the immunological reactivity of Salmonella typhimurium lipopolysaccharide (LPS) was studied. Hydrolysis of LPS at 37 C in 0.01 to 0.25 n NaOH caused a decrease in precipitation of LPS by antiserum. Red cells sensitized with alkali-treated LPS were less responsive to hemagglutination by antiserum than were cells sensitized with heated LPS. Hemagglutination-inhibition studies showed that alkali-treated S. typhimurium LPS was antigenically deficient. Alkaline hydrolysis of S. typhimurium LPS destroys antigen 5 of the Kauffmann-White scheme by cleavage of O-acetyl groups. However, abequose, the immunodominant sugar for antigen 4, is also cleaved from the O side chains by alkali, suggesting the loss of more than one antigenic determinant. It is concluded that alkali treatment of LPS to promote the sensitization of red cells may alter the serological specificity and therefore places limitations on its use in serological studies.

机译：研究了轻度碱性水解对鼠伤寒沙门氏菌脂多糖（LPS）免疫反应的影响。 LPS在37℃下于0.01至0.25 n NaOH中的水解会导致LPS的抗血清沉淀减少。与经加热的LPS致敏的细胞相比，经碱处理的LPS致敏的红细胞对抗血清的血凝反应的反应性更低。血凝抑制研究表明，碱处理的 S。鼠伤寒脂多糖缺乏抗原。 S的碱水解。鼠伤寒 LPS通过裂解 O -乙酰基破坏了Kauffmann-White方案的抗原5。但是，适量的抗原4的免疫主要糖也被碱从 O 侧链上切割掉，这表明失去了多个抗原决定簇。结论是，碱处理LPS以促进红细胞敏化可能会改变血清学特异性，因此限制了其在血清学研究中的使用。 展开▼

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《Infection and immunity》 |1970年第5期|共7页

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Ivan ?i?nár; J. W. Shands;
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中图分类医学免疫学;

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1. Acetylation (O-factor 5) affects the structural and immunological properties of Salmonella typhimurium lipopolysaccharide O antigen. [J] . J M Slauch, M J Mahan, P Michetti, Infection and immunity . 1995,第2期

机译：乙酰化（O因子5）影响鼠伤寒沙门氏菌脂多糖O抗原的结构和免疫学特性。

2. Ornithine transcarbamylase from Salmonella typhimurium: purification, subunit composition, kinetic analysis, and immunological cross-reactivity. [J] . A T Abdelal, E H Kennedy, O Nainan Journal of bacteriology . 1977,第3期

机译：鼠伤寒沙门氏菌的鸟氨酸转氨甲酰酶：纯化，亚基组成，动力学分析和免疫学交叉反应。

3. Regulated delayed synthesis of lipopolysaccharide and enterobacterial common antigen of Salmonella Typhimurium enhances immunogenicity and cross-protective efficacy against heterologous Salmonella challenge [J] . Huang Chun, Liu Qing, Luo Yali, Vaccine . 2016,第36期

机译：鼠伤寒沙门氏菌的脂多糖和肠细菌共同抗原的调控延迟合成可增强针对异源沙门氏菌攻击的免疫原性和交叉保护功效

4. Evaluation of Salmonella Typhimurium fecal shedding in pigs vaccinated with Salmonella Choleraesuis-Typhimurium vaccine via drinking water and challenged four weeks later [C] . Q. Steichen, R. A. Smiley, B. Fergen, Annual^Meeting of the American^Association^of^Swine^Veterinarians. . 2016

机译：评价猪沙门氏菌育鼠粪便患者在饮用水中用沙门氏菌胆管疫苗疫苗接种疫苗，并挑战四周后

5. The role of lipopolysaccharide in the development of specific immunity to Salmonella typhimurium. [D] . Zirk, Nicole Marie. 2000

机译：脂多糖在鼠伤寒沙门氏菌特异性免疫发展中的作用。

6. Effect of Alkali on the Immunological Reactivity of Lipopolysaccharide from Salmonella typhimurium [O] . Ivan Čižnár, J. W. Shands Jr. 1970

机译：碱对鼠伤寒沙门氏菌脂多糖免疫活性的影响

7. Effect of Alkali on the Immunological Reactivity of Lipopolysaccharide from Salmonella typhimurium [O] . Ivan Čižnár, J. W. Shands 1970

机译：碱对沙门氏菌沙门氏菌脂多糖免疫反应性的影响

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3. A O- The separation method of anti-inflammatory lipid A and immunostimulatory core O-antigen polysaccharides from lipopolysaccharide of Salmonella Typhimurium mutation strain [P] . 外国专利： KR20210083430A . 2021-07-07

机译：一种o-抗炎性脂质A和免疫刺激性核心O-抗原多糖的分离方法来自沙门氏菌突变突变菌株的脂多糖

4. typhimurium Salmonella enterica serotype 1.2 having cross reactivity with Salmonella enterica serotype 4,5,12: a specific monoclonal antibody against antigen h i: - producing cell line and its use. [P] . 外国专利： ES2349204B1 . 2011-11-15

机译：鼠伤寒沙门氏菌血清型1.2与肠炎沙门氏菌血清型4,5,12具有交叉反应性：一种针对产生抗原的细胞株的特异性单克隆抗体及其用途。

5. A monoclonal antibody specific to antigen h: 1,2 of salmonella enterica serotype typhimurium that presents cross reactivity with salmonella enterica serthype 4,5,12: i: -, cellular line producer, and its use. (Machine-translation by Google Translate, not legally binding) [P] . 外国专利： ES2349204A1 . 2010-12-29

机译：对肠沙门氏菌血清型鼠伤寒沙门氏菌抗原h：1,2具有特异性的单克隆抗体，可与沙门氏菌肠型4,5,12：i：-交叉反应，细胞系生产商及其用途。（通过Google翻译进行机器翻译，没有法律约束力）

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Effect of Alkali on the Immunological Reactivity of Lipopolysaccharide from Salmonella typhimurium

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