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首页> 外文期刊>Infection and immunity >Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Characterization of the Experimental System
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Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Characterization of the Experimental System

机译：接种牛分枝杆菌卡介苗细胞壁的小鼠细胞的巨噬细胞迁移抑制研究：实验系统的表征。

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The macrophage migration inhibition test was applied to the study of delayed hypersensitivity in mice vaccinated intravenously with oil-treated cell walls of Mycobacterium bovis BCG. Migration inhibition of peritoneal exudate cells from sensitized mice was demonstrated directly upon incubation of the cells with purified protein derivative, but indicator cells such as normal peritoneal cells had to be included to demonstrate migration and migration inhibition with sensitized lung cells. Inhibition of migration induced by mouse cells was greatest 3 to 4 weeks after sensitization but was still considerable after 11 weeks. The migration inhibitory factor (MIF) was not detected in cells freshly isolated from sensitized mice but was released into the supernatant fluid when cells were incubated with purified protein derivative for 24 hr at 37 C in a tissue culture system. Production of MIF was inhibited by actinomycin D and puromycin. MIF was nondialyzable, resistant to heating at 56 C for 1 hr, and of a lower molecular weight than mouse gamma globulin. All data indicated that migration inhibition induced by cells from cell wall-vaccinated mice was very similar to that caused by guinea pig lymphocytes.
机译：巨噬细胞迁移抑制试验用于研究用牛分枝杆菌BCG油处理的细胞壁静脉注射疫苗的小鼠的迟发型超敏反应。在将细胞与纯化的蛋白衍生物孵育后，直接证明了致敏小鼠腹膜渗出液的迁移抑制作用，但是必须包括指示性细胞（如正常腹膜细胞）以证明致敏肺细胞的迁移和迁移抑制作用。致敏后3至4周，小鼠细胞诱导的迁移抑制作用最大，但11周后仍相当可观。在从敏化小鼠新鲜分离的细胞中未检测到迁移抑制因子（MIF），但在组织培养系统中将细胞与纯化的蛋白衍生物在37°C下孵育24小时后，迁移抑制因子释放到上清液中。 MIF的产生被放线菌素D和嘌呤霉素抑制。 MIF是不可透析的，在56°C的温度下仍能抵抗1个小时的加热，并且分子量低于小鼠γ球蛋白。所有数据表明，细胞壁接种的小鼠的细胞诱导的迁移抑制与豚鼠淋巴细胞引起的迁移抑制非常相似。

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