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首页> 外文期刊>Infection and immunity >Host Defense Mechanisms Against Infectious Bovine Rhinotracheitis Virus: In Vitro Stimulation of Sensitized Lymphocytes by Virus Antigen
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Host Defense Mechanisms Against Infectious Bovine Rhinotracheitis Virus: In Vitro Stimulation of Sensitized Lymphocytes by Virus Antigen

机译：牛传染性鼻气管炎病毒的宿主防御机制：通过病毒抗原体外刺激敏感的淋巴细胞。

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Isolated peripheral blood lymphocytes (PBL) from cattle immunized or infected with infectious bovine rhinotracheitis (IBR) virus were cultured in vitro with ultraviolet light-inactivated IBR virus, and the degree of lymphocyte blastogenesis was quantitated by measurement of the uptake of [3H]thymidine into acid-insoluble material. Lymphocyte blastogenesis only occurred with PBL from immunized or infected animals. The optimal conditions for lymphocyte blastogenesis were defined. Blastogenesis was specific since cells from animals immunized against IBR failed to react with two other herpesvirus antigens tested, herpes simplex and equine rhinopneumonitis viruses. Blastogenesis could be prevented by reacting IBR antigen with IBR-specific antibody before adding to cultures, but incorporating IBR-specific antibody in the culture medium after adding free antigen failed to inhibit blastogenesis. With intranasally infected animals, lymphocyte blastogenesis was detectable after 5 days, reached peak levels between days 7 and 10, and then declined to low levels by day 19. In contrast, levels of neutralizing antibody were barely detectable on day 7 and reached maximal concentrations on day 19. The lymphocyte blastogenesis assay was emphasized as a convenient and useful in vitro correlate of cell-mediated immunity that should help define the role of cell-mediated immunity in immunity to herpesviruses.
机译：用紫外线灭活的IBR病毒在体外培养免疫或感染牛传染性鼻气管炎（IBR）病毒的牛的外周血淋巴细胞（PBL），并通过测量[ 3 H]胸苷为不溶于酸的物质。淋巴细胞的成胚作用仅发生于来自免疫或感染动物的PBL。确定了淋巴细胞成胚的最佳条件。由于来自针对IBR免疫的动物的细胞无法与另外两种测试的疱疹病毒抗原（单纯疱疹病毒和马鼻肺炎病毒）发生反应，因此其成胚作用具有特异性。可以通过在添加到培养物中之前使IBR抗原与IBR特异性抗体反应来防止成纤维细胞形成，但是在添加游离抗原后将IBR特异性抗体掺入培养基中不能抑制成纤维细胞形成。对于鼻内感染的动物，可在5天后检测到淋巴细胞的成骨作用，并在第7天至第10天达到峰值，然后在第19天下降至低水平。相反，在第7天几乎无法检测到中和抗体的水平，并在第7天达到最大浓度。第19天，强调了淋巴细胞的成生测定是细胞介导的免疫的方便和有用的体外相关性，其应有助于确定细胞介导的免疫在对疱疹病毒的免疫中的作用。

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