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Binding of cholera toxin by various tissues.

机译:霍乱毒素被各种组织结合。

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Under certain conditions, it is possible to confirm the observation by Peterson (1974) that the cholera toxin-binding capacities of tissues from brain and colon mucosa, and from liver and small intestine mucosa, are comparable. Binding of toxin by all tissues except brain is very variable, but is roughtly proportional to their content of the toxin-binding ganglioside galactosyl-N-acetylgalactosaminyl (sialosyl) lactosyl ceramide. It appears that some toxin-binding sites of the mucosa of the small intestin and colon may be masked. It has also been confirmed that there may be some solubilization of toxin-binding material from brain on standing a few days at 4 C, but this is comparatively slight. Some disadvantages of measuring toxin binding by adding small amounts of radioactive toxin to compartively large amounts of tissue are discussed.
机译:在某些条件下,有可能证实Peterson(1974)的观察结果,即脑和结肠粘膜以及肝脏和小肠粘膜组织的霍乱毒素结合能力是可比的。除大脑外,所有组织的毒素结合都是非常可变的,但与它们与毒素结合神经节苷脂半乳糖基-N-乙酰半乳糖胺基(唾液酰基)乳糖基神经酰胺的含量大致成比例。看来小肠和结肠粘膜的一些毒素结合位点可能被掩盖了。还已经证实,在4°C放置几天后,可能会从大脑中溶解一些毒素结合物质,但这相对较少。讨论了通过向相对大量的组织中添加少量放射性毒素来测量毒素结合的一些缺点。

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