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首页> 外文期刊>Infection and immunity >Depression of Rauscher leukemia virus envelope glycoprotein gp71 binding by lymphoid cells during leukemogenesis in mice.
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Depression of Rauscher leukemia virus envelope glycoprotein gp71 binding by lymphoid cells during leukemogenesis in mice.

机译：小鼠白血病形成过程中淋巴样细胞抑制罗氏白血病病毒包膜糖蛋白gp71结合。

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The availability of membrane receptors for the 71,000-dalton envelope glycoprotein (gp71) of Rauscher murine leukemia virus on splenic and thymic cells from BALB/c mice during Rauscher murine leukemia virus-induced leukemogenesis was determined utilizing a radiolabeled gp71 binding assay. Shortly after infection, the relative cellular [125I]gp71 binding level decreased, first with splenic cells (at day 7 to 10 after infection) and later with thymic cells (at day 10 to 20 after infection). The dependency of the reduction of binding on the replication of the inoculated virus was demonstrated by regression analyses using cellular gp71 binding level as the dependent variable and infectious virus titer, as well as viral gp71 and p30 levels, of spleens and thymuses from infected mice as independent variables. With each independent variable, the reduction of gp71 binding for both cell types was highly dependent (P less than 0.01) on the level of virus detected in their respective organ. In the early stages of leukemogenesis, the [125I]gp71 binding level declined to approximately 20 to 30% of control values. During this period the rate of reduction of binding was very rapid and, in general was similar for both splenic and thymic cells. Further progression of the disease resulted in little or no further reduction in binding. The application of this technique to monitor host ecotropic virus synthesis and to study cell surface virus receptor control mechanisms in vivo is discussed.
机译：利用放射性标记的gp71结合测定法确定了劳氏鼠白血病病毒诱导的白血病发生过程中BALB / c小鼠脾脏和胸腺细胞上的劳氏鼠白血病病毒71,000道尔顿包膜糖蛋白（gp71）的膜受体的可用性。感染后不久，相对细胞[125I] gp71结合水平降低，首先是脾细胞（感染后第7至10天），然后是胸腺细胞（感染后第10至20天）。通过使用细胞gp71结合水平作为因变量和传染性病毒效价以及感染小鼠脾脏和胸腺的病毒gp71和p30水平的回归分析，证明了结合减少对接种病毒复制的依赖性。自变量。对于每个独立变量，两种细胞类型中gp71结合的减少高度依赖（P小于0.01）取决于它们各自器官中检测到的病毒水平。在白血病发生的早期阶段，[125I] gp71结合水平下降至对照值的约20％至30％。在此期间，结合的减少速率非常快，并且对于脾细胞和胸腺细胞而言，通常是相似的。疾病的进一步发展导致结合的减少很少或没有。讨论了该技术在监测宿主嗜性病毒合成和研究体内细胞表面病毒受体控制机制中的应用。

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