...
首页> 外文期刊>Infection and immunity >Contribution of Humoral and Cellular Factors to the Resistance to Experimental Infection by Pseudomonas aeruginosa in Mice I. Interaction Between Immunoglobulins, Heat-Labile Serum Factors, and Phagocytic Cells in the Killing of Bacteria
【24h】

Contribution of Humoral and Cellular Factors to the Resistance to Experimental Infection by Pseudomonas aeruginosa in Mice I. Interaction Between Immunoglobulins, Heat-Labile Serum Factors, and Phagocytic Cells in the Killing of Bacteria

机译:体液和细胞因子对小鼠铜绿假单胞菌对实验感染的抗性的贡献I.免疫球蛋白,热不稳定血清因子和吞噬细胞在细菌杀死中的相互作用

获取原文
           

摘要

Techniques have been described for the purification of mouse polymorphonuclear leukocytes (PMN) and macrophages and for determining their bactericidal activity against Pseudomonas aeruginosa in a rotating suspension. Requirements for human anti-Pseudomonas opsonins and heat-labile mouse serum factors have been determined. In the macrophage system, although heat-labile mouse serum factors had enhancing properties, both immunoglobulin G (IgG) and immunoglobulin M (IgM) opsonins alone sufficed to induce bactericidal activity. In contrast, both opsonins and heat-labile mouse serum factors were required for bactericidal activity by the mouse PMN. In the absence of heat-labile mouse serum factors, IgG and IgM opsonins contributed to the bactericidal activity of mouse macrophages to the same extent. However, in the presence of these factors, much less IgG than IgM antibody was required to achieve maximum bactericidal activity by these cells. Similarly, IgG opsonins were found to be more efficient than IgM opsonins in enhancing the killing of P. aeruginosa by mouse PMN.
机译:已经描述了用于纯化小鼠多形核白细胞(PMN)和巨噬细胞并确定其对旋转悬浮液中铜绿假单胞菌的杀菌活性的技术。已经确定了人类抗假单胞菌调理素和热不稳定小鼠血清因子的需求。在巨噬细胞系统中,尽管热不稳定的小鼠血清因子具有增强的特性,但单独的免疫球蛋白G(IgG)和免疫球蛋白M(IgM)调理素都足以诱导杀菌活性。相反,小鼠PMN的杀菌活性需要调理素和不耐热的小鼠血清因子。在不存在热不稳定的小鼠血清因子的情况下,IgG和IgM调理素在相同程度上促进了小鼠巨噬细胞的杀菌活性。但是,在存在这些因素的情况下,要获得这些细胞的最大杀菌活性,所需的IgG比IgM抗体少得多。类似地,发现IgG调理素在增强 P的杀伤方面比IgM调理素更有效。小鼠PMN引起铜绿。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号