In Vitro Uptake of 14C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies

R. W. Johns; G. T. Dimopoullos

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In Vitro Uptake of 14C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies

机译：通过部分纯化的无浆无浆体的体外吸收14C标签的氨基酸。

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Studies on the incorporation of three 14C-labeled amino acids into preparations of partially purified Anaplasma marginale were conducted. Relatively high levels of uptake of radioactive isoleucine, glycine, and methionine were detected in both trichloroacetic acid-soluble and -insoluble fractions of these preparations, indicating that the parasite is capable of protein synthesis outside its erythrocytic environment. When erythrocytes (red blood cells) from infected calves were incubated with [14C]methionine and Anaplasma bodies were subsequently purified, it was found that some of the label detected was associated with the marginal bodies.

机译：进行了将3个 14 C标记氨基酸掺入部分纯化的 Anaplasma marginale 制剂中的研究。在这些制剂的三氯乙酸可溶和不可溶部分中均检测到相对较高水平的放射性异亮氨酸，甘氨酸和蛋氨酸吸收，表明该寄生虫能够在其红细胞环境之外进行蛋白质合成。将感染小牛的红细胞与[ 14 C]蛋氨酸一起温育并随后纯化质膜体时，发现检测到的某些标记物是相关的与边缘的身体。 展开▼

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《Infection and immunity》 |1974年第4期|共3页

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R. W. Johns; G. T. Dimopoullos;
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中图分类医学免疫学;

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1. In Vitro Uptake of 14C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies [J] . R. W. Johns, G. T. Dimopoullos Infection and immunity . 1974,第4期

机译：通过部分纯化的无浆无浆体的体外吸收14C标签的氨基酸。

2. Comparative Study of 14C-Labeled Purified Protein Derivative from Various Mycobacteria I. Preparation of 14C-Labeled Purified Protein Derivative Antigens and Their Adsorption to Glass [J] . S. Landi, H. R. Held, M. C. Tseng Applied Microbiology . 1970,第5期

机译：来自各种分枝杆菌的14C标签纯化蛋白衍生物的比较研究I. 14C标签纯化蛋白衍生物抗原的制备及其对玻璃的吸附

3. Comparative Study of 14C-Labeled Purified Protein Derivative from Various Mycobacteria I. Preparation of 14C-Labeled Purified Protein Derivative Antigens and Their Adsorption to Glass [J] . S. Landi, H. R. Held, M. C. Tseng Applied Microbiology . 1970,第5期

机译：来自各种分枝杆菌的14C标签纯化蛋白衍生物的比较研究I. 14C标签纯化蛋白衍生物抗原的制备及其对玻璃的吸附

4. Catalytic Asymmetric Synthesis of α-Amino Acid Derivatives: Approaches toward Green Sustainable Methodology for Preparation of Optically Active Amino Acids [C] . Yasuhiro Yamashita, Shu Kobayashi Asymmetric synthesis and application of α-amino acids . 2007

机译：催化不对称合成α-氨基酸衍生物：绿色可持续方法制备光学活性氨基酸的方法

5. PREPARATION AND USE OF BOVINE JEJUNAL BRUSH BORDER MEMBRANE VESICLES TO INVESTIGATE AMINO ACID UPTAKE (NUTRIENT, RUMINANT, ABSORPTION, CATTLE, TRANSPORT). [D] . CROOKER, BRIAN ALWYN. 1985

机译：牛幼肠刷膜膜囊的制备和使用以研究氨基酸摄取（营养，反刍动物，吸收，牛，运输）。

6. In Vitro Uptake of 14C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies [O] . R. W. Johns, G. T. Dimopoullos 1974

机译：通过部分纯化的无浆无浆体的体外吸收14C标签的氨基酸。

7. In Vitro Uptake of 14 C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies [O] . R. W. Johns, G. T. Dimopoullos 1974

机译：通过部分纯化的环脂体骨体体进行体外吸收14个C标记的氨基酸

8. Plant Uptake of 2,4,6-Trinitrotoluene, 4-Amino-2,6-Dinitrotoluene, and 2-Amino-4,6-Dinitrotoluene Using 14C-Labeled and Unlabeled Compounds [R] . Pennington, J. C. 1988

机译：植物摄取2,4,6-三硝基甲苯，4-氨基-2,6-二硝基甲苯和2-氨基-4,6-二硝基甲苯使用14C标记和未标记化合物

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2. 边缘无浆体MSP5基因序列分析及其融合蛋白的纯化 [J] . 马米玲 ,罗建勋 ,殷宏 . 动物医学进展 . 2008,第008期

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1. 一种温和无刺激的氨基酸纯化及提取工艺 [P] . 中国专利： CN111943861A . 2020-11-17

2. 中医无创体外智能快检系统及中医无创体外智能快检装置 [P] . 中国专利： CN109431469A . 2019-03-08

3. Procedure for obtaining Antigen of Anaplasma marginale, useful in the preparation of Vaccines for cattle [P] . 外国专利： MX166157B . 1992-12-22

机译：获得无缘无浆膜抗原的程序，可用于牛疫苗的制备

4. Process for the preparation of a composition for anaplasmosis specific antigen of Anaplasma marginale and comprising a water soluble Antigen and a corpuscular Antigen [P] . 外国专利： MX7005E . 1987-01-30

机译：制备用于无缘无水质子的无病菌特异性抗原的组合物的方法，所述组合物包含水溶性抗原和小体抗原

5. Use of 13C or 14C-labeled amino acids or peptides as diagnostic agents for exocrine pancreatic function [P] . 外国专利： DE69927089T2 . 2006-05-18

机译：使用13C或14C标记的氨基酸或肽作为外分泌胰腺功能的诊断剂

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In Vitro Uptake of 14C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies

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