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首页> 外文期刊>Infection and immunity >Comparison of serological tests for antibody to hepatitis A antigen, using coded specimens from individuals infected with the MS-1 strain of hepatitis A virus.
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Comparison of serological tests for antibody to hepatitis A antigen, using coded specimens from individuals infected with the MS-1 strain of hepatitis A virus.

机译：使用感染甲型肝炎病毒MS-1株的个体的编码标本比较抗甲型肝炎抗体的血清学测试。

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To compare serological tests for antibody to hepatitis A antigen (anti-HA), we tested 15 paired serum specimens, submitted under code, from individuals infected with the MS-1 strain of hepatitis A virus. Immune electron microscopy (IEM), immune adherence hemagglutination (IAHA), and solid-phase radioimmunoassay (RIA) tests for anti-HA were performed with hepatitis A antigen (HA Ag) derived from human stool; results were also compared with previously reported titers determined by IAHA with HA Ag derived from marmoset liver. Antibody titers (IAHA and RIA) and ratings (IEM) determined with stool-derived HA Ag compared favorably, and a seroresponse to HA Ag was detected by all three methods for every serum pair tested. Differences in titers were noted between IAHA tests with liver-derived and with stool-derived HA Ag, but the discrepancies could be accounted for by differences in test technique. The agreement found in this study among the three techniques was quite good and confirms the specificity and sensitivity of tests for anti-HA that are done with stool-derived HA-Ag.
机译：为了比较针对甲型肝炎抗原（anti-HA）抗体的血清学测试，我们测试了15对配对的血清标本，这些标本是根据代码提交的，感染了甲型肝炎病毒MS-1株的个体。用源自人类粪便的甲型肝炎抗原（HA Ag）进行了抗HA的免疫电子显微镜（IEM），免疫粘附血凝（IAHA）和固相放射免疫法（RIA）测试。还将结果与IAHA用mar猴肝脏来源的HA Ag测定的先前报道的效价进行了比较。用粪便来源的HA Ag测定的抗体滴度（IAHA和RIA）和等级（IEM）比较好，并且对于所有测试的血清对，通过所有三种方法检测到对HA Ag的血清反应。 IAHA试验在肝源性和粪便源性HA Ag之间的效价存在差异，但差异可能是由于测试技术的差异所致。在这项研究中，这三种技术之间的一致性非常好，并证实了粪便来源的HA-Ag对抗HA的检测的特异性和敏感性。

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