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首页> 外文期刊>Infection and immunity >Identification of cholesterol in the receptor site for rickettsiae on sheep erythrocyte membranes.
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Identification of cholesterol in the receptor site for rickettsiae on sheep erythrocyte membranes.

机译：鉴定绵羊红细胞膜上立克次体的受体位点中的胆固醇。

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摘要

Cholesterol was identified as an essential component of the receptor on the sheep erythrocyte to which Rickettsia prowazeki adsorbs before lysing the cell. Erythrocytes or ghosts, derived by hypotonic lysis, were treated with proteolytic enzymes, sialidase, sulfhydryl reagents, and periodate without affecting their ability to adsorb rickettsiae. Lipid extracts of ghosts and erythrocytes, on the other hand, contained receptor activity. Fractionation of the lipid extracts by silicic acid column chromatogrphy resulted in the isolation of receptor activity in a neutral lipid fraction. The lipid fractions demonstrated receptor acitity at 34 C but not at 0 C. These properties are also characteristic of the receptor activity with erythrocytes and ghosts. Cholesterol, co-lyophilized with palmitic acid, was found to possess receptor activity. Palmitic acid alone, cholesterol-lecithin, cholestane-palmitic acid, and various phospholipids and glycolipids had no receptor activity. Ghosts treated with amphotericin B or digitonin, compounds that bind to cholesterol in the membrane, lost their ability to adsorb rickettsiae.
机译：胆固醇被鉴定为羊红细胞上受体的必需成分，原红立克次氏体在裂解细胞之前就被吸收。通过蛋白水解酶，唾液酸酶，巯基试剂和高碘酸盐处理低渗裂解产生的红细胞或鬼影，而不会影响它们吸收立克次体的能力。另一方面，鬼和红细胞的脂质提取物具有受体活性。通过硅酸柱色谱法分离脂质提取物可分离中性脂质馏分中的受体活性。脂质级分在34°C时表现出受体活性，而在0°C时则没有。这些特性也是红细胞和鬼影受体活性的特征。与棕榈酸共冻干的胆固醇具有受体活性。单独的棕榈酸，胆固醇-卵磷脂，胆甾烷-棕榈酸以及各种磷脂和糖脂都没有受体活性。用两性霉素B或洋地黄皂苷（与膜中的胆固醇结合的化合物）处理过的鬼魂失去了吸附立克次体的能力。

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