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Nature of the genetic determinant controlling encapsulation in Staphylococcus aureus Smith.

机译:控制金黄色葡萄球菌史密斯封装的遗传决定因素的性质。

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Two strains of Staphylococcus aureus, Smith and M, were studied for the elimination of encapsulation. For S. aureus M, encapsulation was stable. For S. aureus Smith, spontaneous loss of encapsulation was 1.3% and increased markedly in medium containing surface-active agents. In the presence of sodium dodecyl sulfate, unencapsulated cells had a considerable selective advantage. Attempts to demonstrate covalently closed circular plasmid deoxyribonucleic acid were unsuccessful. In cultures of unencapsulated cells, encapsulated cells were observed occasionally. These data argue against a plasmid location for the determinants controlling encapsulation in this organism in spite of a high spontaneous loss of this character.
机译:研究了两种金黄色葡萄球菌菌株Smith和M,用于消除包囊。对于金黄色葡萄球菌M,包封是稳定的。对于金黄色葡萄球菌史密斯,自发的包封损失为1.3%,在含有表面活性剂的培养基中明显增加。在十二烷基硫酸钠的存在下,未封装的细胞具有相当大的选择性优势。试图证明共价闭合的环状质粒脱氧核糖核酸是不成功的。在未包囊细胞的培养物中,偶尔观察到包囊细胞。这些数据与质粒位置无关,尽管该位置自发性丧失很高,但质粒位置决定了控制该生物体中包囊的决定因素。

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