The effect of oral administration of soluble antigen preparations containing glucosyltransferase on dental caries in hamsters was studied. Immunization was accomplished by feeding glucosyltransferase for 21 to 27 consecutive days. This immunization regimen resulted in the formation of salivary antibody, which was detected by functional inhibition of enzymatic activity and by a modified enzyme-linked immunosorbent assay. A serum response also occurred in two of the three experiments performed. After infection with cariogenic Streptococcus mutans strain 6715, glucosyltransferase-fed hamsters had significantly fewer S. mutans cells recoverable from molar surfaces on six of nine occasions, compared with buffer-fed control groups. Hamsters orally immunized with glucosyltransferase also always had lower mean caries scores and mean numbers of lesions than comparably infected sham-immunized groups. The results of this study suggest that significant protection from experimental dental caries can be accomplished by oral administration of soluble antigen preparations containing glucosyltransferase.
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