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Unique lipid composition of Treponema pallidum (Nichols virulent strain).

机译:梅毒螺旋体(Nichols强毒株)的独特脂质成分。

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The lipid composition of Treponema pallidum (Nichols virulent strain) was determined after purification of the organisms from the infected testes of corticosteroid-treated rabbits by differential centrifugation, filtration through Nuclepore membranes, and sedimentation in Hypaque density gradients. The total lipids were comprised of 32.2% neutral lipids, mainly cholesterol, and 67.8% phospholipids consisting of phosphatidylcholine (32.1%), sphingomyelin (14.8%), cardiolipin (13.0%), phosphatidylethanolamine (6.2%), phosphatidylinositol-serine (1.2%), and lysophosphatidylcholine (0.4%). Monoglycosyldiglyceride, a glycolipid comprising 25 to 50% of thetotal lipid of all Treponema previously examined, was not detected. The fatty acid composition was similar but quntitatively distinct from that of the infected testes tissue.
机译:在通过差速离心,通过核孔膜过滤以及在Hypaque密度梯度中沉降从感染的皮质类固醇治疗的兔的睾丸中纯化生物体后,测定了梅毒螺旋体(Nichols毒株)的脂质组成。总脂质包括32.2%的中性脂质(主要是胆固醇)和67.8%的磷脂,包括磷脂酰胆碱(32.1%),鞘磷脂(14.8%),心磷脂(13.0%),磷脂酰乙醇胺(6.2%),磷脂酰肌醇丝氨酸(1.2%) )和溶血磷脂酰胆碱(0.4%)。未检测到单糖基二甘油酯,一种糖脂,占先前检查的所有密螺旋体总脂质的25%至50%。脂肪酸组成与被感染的睾丸组织相似,但在数量上截然不同。

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