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Development of Bactericidal Capacity and Phagocytosis-Associated Metabolism of Fetal Pig Leukocytes

机译：胎猪白细胞的杀菌能力和吞噬作用相关代谢的发展

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Evidence that the bactericidal ability and the stimulated oxidative metabolism of leukocytes appear in parallel during fetal development of the Minnesota Miniature pig has been obtained by application of the techniques applied to studies of human cells. It was demonstrated that leukocytes from 87- to 90-day fetuses were fully capable of ingesting Staphylococcus aureus but greatly diminished in bactericidal capacity as compared to leukocytes of older fetuses and adults. Although resting levels of oxygen consumption and hexose monophosphate pathway activity of leukocytes from the younger fetuses compared well with those of leukocytes from older animals, the phagocytosis-stimulated increments of metabolism were much less at 87 to 90 days of gestation than at later developmental stages. Both bactericidal capacity and increased metabolism of leukocytes reach adult levels by 100 days of gestation (normal gestation period of 115 to 120 days). Acrylamide gels stained for reduced nicotinamide adenine dinucleotide (NADH) and NADH phosphate (NADPH) diaphorase activity after disc electrophoresis of leukocyte extracts revealed normal mobility and intensity of NADH diaphorase bands. Three NADPH diaphorase bands were present in adult leukocyte extracts. Only the fast-migrating NADPH diaphorase band of 87- to 90-day cells stained with decreased intensity. This “deficiency” was no longer present at the later fetal period. The fast-migrating NADPH diaphorase band may represent an electron transfer protein which functions in cyanide-insensitive respiration of the leukocytes of the pig.
机译：在明尼苏达州小型猪的胎儿发育过程中，白细胞的杀菌能力和刺激的氧化代谢同时出现的证据已通过应用用于人体细胞研究的技术获得。结果表明，与年龄较大的胎儿和成年人的白细胞相比，从87天到90天的胎儿的白细胞具有充分的摄取金黄色葡萄球菌的能力，但杀菌能力却大大降低。尽管年轻胎儿白细胞的静息耗氧量和己糖单磷酸途径活性与老年动物白细胞的静息相比很好，但吞噬作用刺激的新陈代谢在妊娠87至90天时要比在后期发育阶段低得多。妊娠100天（正常妊娠期115至120天）的杀菌能力和白细胞新陈代谢都达到成人水平。在白细胞提取物的圆盘电泳显示出正常的迁移率和强度的NADH心肌磷脂酶带后，对减少的烟酰胺腺嘌呤二核苷酸（NADH）和NADH磷酸盐（NADPH）心肌磷脂酶活性进行染色的丙烯酰胺凝胶。成人白细胞提取物中存在三个NADPH心肌黄递酶带。仅快速迁移的87至90天细胞的NADPH心肌黄递酶条带染色强度降低。在胎儿后期不再出现这种“缺陷”。快速迁移的NADPH心肌黄酶带可能代表一种电子转移蛋白，在猪白细胞对氰化物的不敏感呼吸中起作用。

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