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首页> 外文期刊>Infection and immunity >Yeast- and Mycelial-Phase Antigens of Blastomyces dermatitidis: Comparison Using Disc Gel Electrophoresis
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Yeast- and Mycelial-Phase Antigens of Blastomyces dermatitidis: Comparison Using Disc Gel Electrophoresis

机译：皮肤细菌芽孢杆菌酵母和菌丝体抗原：使用圆盘凝胶电泳的比较。

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摘要

The disc gel electrophoretic patterns obtained with skin test-active (mycelial and yeast) antigens of Blastomyces dermatitidis were compared. As would be expected, the blastomycins (mycelial-phase) and the cytoplasmic ultrafiltrates (yeast-phase) were heterogeneous mixtures containing proteins, glycoproteins, lipoproteins, and carbohydrates. The skin test-active cytoplasmic ultrafiltrates and the blastomycins contained glycoproteins that had similar Rf values which allows the possibility that one or more of these components is responsible for the skin-test reactivity of these antigens. The electrophoretic migration of the alkali-soluble, water-soluble cell wall antigen differed from those of the cytoplasmic antigens and the two blastomycins. Electrophoresis, Sephadex chromatography, and ultrafiltration studies showed that the alkali-soluble, water-soluble cell wall antigen is comprised of lipid, polysaccharide, and protein and has a molecular weight range of 30,000 to 50,000. The increased number and mobility of both the protein and carbohydrate bands after denaturation and electrophoresis of this antigen in sodium dodecyl sulfate indicate that there are several cross-linkages between the polysaccharide and/or protein moieties, possibly via lipid or disulfide bridges.
机译：比较了皮肤发炎芽孢杆菌皮肤试验活性（菌丝和酵母）抗原获得的盘状凝胶电泳图谱。可以预料，芽孢霉素（菌丝期）和细胞质超滤液（酵母期）是含有蛋白质，糖蛋白，脂蛋白和碳水化合物的异质混合物。皮肤试验活性细胞质超滤液和母霉素含有的糖蛋白具有相似的 R f 值，这使得这些成分中的一种或多种可能负责皮肤测试这些抗原的反应性。碱溶性，水溶性细胞壁抗原的电泳迁移不同于细胞质抗原和两种母霉素的电泳迁移。电泳，Sephadex色谱和超滤研究表明，碱溶性，水溶性细胞壁抗原由脂质，多糖和蛋白质组成，分子量范围为30,000至50,000。在十二烷基硫酸钠中对该抗原进行变性和电泳后，蛋白质和碳水化合物带的数量和迁移率均增加，这表明多糖和/或蛋白质部分之间可能通过脂质或二硫键桥接了一些交联。

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