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首页> 外文期刊>Infection and immunity >Deregulation of mouse antibody-forming cells in vivo in cell culture by streptococcal pyrogenic exotoxin.
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Deregulation of mouse antibody-forming cells in vivo in cell culture by streptococcal pyrogenic exotoxin.

机译：链球菌热原性外毒素在细胞培养物中体内抑制小鼠抗体形成细胞。

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An unregulated, elevated rebound of antibody levels in rabbits was shown to follow late (10 to 15 days) after steptococcal pyrogenic exotoxin (SPE)-induced immunosuppression. Because of that result we have suggested that SPE acts by preferentially inhibiting a regulatory cell which normally limits the extent of full expression of antibody formation by B-cells. We are currently testing this hypothesis in mice. NIH (Swiss Webster) mice (+/+) or NIH (Swiss Webster) mice heterozygous (+u) for the mutant athymic nude gene and phenotypically normal showed an elevated plaque-forming cell (PFC) response to sheep erythrocytes (SE) late (10 to 15 days) after immunosuppressive SPE treatment similar to that described in rabbits. Homozygous nude mice (nuu) that are phenotypically athymic normally show a reduced early (4 day) PFC response to SE (a T-cell-dependent antigen) as compared with +u littermates or +/+ parent strain mice. This cryptic early 4-day response was improved by injection of purified endotoxin (a B-cell mitogen), but these relatively elevated nude PFC responses had decreased to normal control (SE only)nude PFC levels before 10 days. In similar SE-injected nude mice treated instead with SPE, no elevation at 4 days was observed and, more pertinently, the late (10 to 15 day) elevated rebound of PFC levels observed in normal response controls (+u or +/+) was not observed. Similar experiments were subsequently conducted in Marbrook-type spleen PFC cultures during periods of 12 days. The results of these experiments paralleled the in vivo results above, and in addition showed that SPE induced a large proliferation of either +/+ or +u cells (T-and B-cells) in culture but had no such effect on nuu cells (B-cells) in culture. Purified endotoxin, the Bcell mitogen, had a better sparing effect on nuu cells in this respect. These results are consistent with our premise that SPE inhibits preferentially the function of a regulator of the antibody response. The regulator appears to be a T-cell and is likely a suppressor T-cell.
机译：在兔链球菌热原性外毒素（SPE）诱导的免疫抑制后（10至15天），兔子体内抗体水平的反弹未受调节，升高。由于该结果，我们建议SPE通过优先抑制正常情况下限制B细胞完全表达抗体形成的调节细胞来发挥作用。我们目前正在小鼠中测试这一假设。 NIH（Swiss Webster）小鼠（+ / +）或NIH（Swiss Webster）小鼠的突变无胸腺裸基因杂合（+ / nu），表型正常显示对绵羊红细胞（SE）的噬菌斑形成细胞（PFC）响应升高免疫抑制SPE处理后的晚期（10至15天）类似于兔子中描述的方法。表型无胸腺的纯合裸鼠（nu / nu）与+ / nu同窝小鼠或+ / +亲本小鼠相比，对SE（T细胞依赖性抗原）的PFC应答正常（4天）减少。通过注射纯化的内毒素（B细胞有丝分裂原）可以改善这种隐秘的早期4天反应，但是这些相对升高的裸PFC反应在10天前已降至正常对照（仅SE）裸PFC水平。在用SPE代替SE注射的相似的SE注射裸鼠中，在4天时未观察到升高，更相关的是，在正常反应对照组（+ / nu或+ / + ）没有被观察到。随后在12天的Marbrook型脾PFC培养物中进行了类似的实验。这些实验的结果与上述体内结果相符，此外还显示SPE诱导培养中+ / +或+ / nu细胞（T细胞和B细胞）的大量增殖，但对nu /没有影响培养中的nu细胞（B细胞）。在这方面，纯化的内毒素B细胞有丝分裂原对nu / nu细胞具有更好的保护作用。这些结果符合我们的前提，即SPE优先抑制抗体应答调节剂的功能。调节物似乎是T细胞，可能是抑制性T细胞。

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