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首页> 外文期刊>Infection and immunity >Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations.
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Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations.

机译：大肠杆菌肠毒素：刺激破细胞制剂中的腺苷酸环化酶。

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摘要

The enterotoxin from cell-free filtrates of the enteropathogenic Escherichia coli strain P-263 was found to stimulate adenylate cyclase activity in broken-cell preparations from myocardial tissue. Particulate and detergent-solubilized fractions from cat heart were incubated with enterotoxin and assayed for adenylate cyclase activity. Adenylate cyclase activity was stimulated by enterotoxin; the extent of stimulation was proportional to the concentration of enterotoxin. The data demonstrate that stimulation of enterotoxin-sensitive adenylate cyclase in this system provides a sensitive in vitro assay, either as an accurate measure of enterotoxin concentration or as an assay for antitoxic titers in sera. A parallel comparison showed that stimulation of fluid production in rabbit intestinal loops by enterotoxin was less sensitive.
机译：发现来自肠道致病性大肠杆菌P-263菌株的无细胞滤液中的肠毒素可刺激心肌组织破细胞制剂中的腺苷酸环化酶活性。将来自猫心的颗粒和洗涤剂溶解的级分与肠毒素一起孵育，并分析腺苷酸环化酶的活性。肠毒素刺激腺苷酸环化酶的活性。刺激程度与肠毒素浓度成正比。数据表明，在该系统中刺激肠毒素敏感的腺苷酸环化酶可提供灵敏的体外测定，可作为肠毒素浓度的准确测定或血清中抗毒性滴度的测定。平行比较显示肠毒素刺激家兔肠loop中液体的产生的敏感性较低。

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